Histone acetylation during the in vitro maturation of bovine oocytes with different levels of competence
Thais P. Pontelo
A , Sarah A. D. Rodrigues B , Taynan S. Kawamoto C , Ligiane O. Leme D , A. C. M. M. Gomes E , Marcio G. Zangeronimo A , Mauricio M. Franco E and Margot A. N. Dode E F
+ Author Affiliations
- Author Affiliations
A Department of Veterinary Medicine, Federal University of Lavras, Lavras, MG 32700-000, Brazil.
B Department of Animal Science, University of Brasilia, Brasilia, DF 70910-900, Brazil.
C Department of Veterinary Medicine, Federal University Uberlândia, Uberlândia, MG 38400-902, Brazil.
D Department of Animal Science, Federal University of Espírito Santo, Vitória, ES 29075-073, Brazil.
E Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-900, Brazil.
F Corresponding author. Email: margot.dode@embrapa.br
Reproduction, Fertility and Development 32(7) 690-696 https://doi.org/10.1071/RD19218
Submitted: 17 June 2019 Accepted: 6 December 2019 Published: 31 March 2020
Abstract
We aimed to analyse the histone acetylation status and expression profile of genes involved in histone acetylation (histone acetyltransferase 1 (HAT1), lysine acetyltransferase 2A (KAT2A), histone deacetylase 1 (HDAC1), HDAC2 and HDAC3) in bovine oocytes of different competences during in vitro maturation (IVM). Cumulus–oocyte complexes were recovered from two groups of follicles: minor follicles (1.0–3.0 mm in diameter), classified as low competence (LC) and large follicles (6.0–8.0 mm in diameter) classified as high competence (HC). Oocytes were submitted to IVM for 0, 8 and 24 h and stored for analysis. Acetylation status of histone H4 on lysine K5, K6, K12 and K16 was assessed by immunohistochemistry. For gene expression, mRNA levels were determined by real-time quantitative polymerase chain reaction. All oocytes, regardless of their competence, showed a gradual decrease (P < 0.05) in acetylation signals during IVM. From 0 to 8 h of maturation, an increase (P < 0.05) in the relative abundance of HAT1 mRNA was observed only in the HC oocytes. In this group, higher (P < 0.05) mRNA levels of HDAC1 at 8 h of maturation were also observed. In conclusion, in the present study, LC oocytes were shown to have adequate acetylation levels for the resumption and progression of meiosis; however, these oocytes do not have the capacity to synthesise RNA during IVM as the HC oocytes do.
Additional keywords: cumulus–oocyte complexes, gene expression, histone acetyltransferase, immunohistochemistry, epigenetic marks.
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