Localisation of phospholipase Cζ1 (PLCZ1) and postacrosomal WW-binding protein (WBP2 N-terminal like) on equine spermatozoa and flow cytometry quantification of PLCZ1 and association with cleavage in vitro
Raul A. Gonzalez-Castro
A , Fabio Amoroso-Sanches A , JoAnne E. Stokes A , James K. Graham A and Elaine M. Carnevale A B
+ Author Affiliations
- Author Affiliations
A Equine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, 3101 Rampart Rd, Fort Collins, Colorado, 80521, USA.
B Corresponding author. Email: elaine.carnevale@colostate.edu
Reproduction, Fertility and Development 31(12) 1778-1792 https://doi.org/10.1071/RD19217
Submitted: 14 June 2019 Accepted: 3 September 2019 Published: 10 October 2019
Abstract
Oocyte activation is initiated when a fertilising spermatozoon delivers sperm-borne oocyte-activating factor(s) into the oocyte cytoplasm. Candidates for oocyte activation include two proteins, phospholipase Cζ1 (PLCZ1) and postacrosomal WW-binding protein (PAWP; also known as WBP2 N-terminal like (WBP2NL)). We localised PLCZ1 and WBP2NL/PAWP in stallion spermatozoa and investigated the PLCZ1 content and sperm parameters as well as cleavage after intracytoplasmic sperm injection (ICSI). PLCZ1 was identified as 71-kDa protein in the acrosomal and postacrosomal regions, midpiece and principal piece of the tail. Anti-WBP2NL antibody identified two WBP2NL bands (~28 and ~32 kDa) in the postacrosomal region, midpiece and principal piece of the tail. PLCZ1 and WBP2NL expression was positively correlated (P = 0.04) in sperm heads. Flow cytometry evaluation of PLCZ1 revealed large variations in fluorescence intensity and the percentage of positively labelled spermatozoa among stallions. PLCZ1 expression was significantly higher in viable than non-viable spermatozoa, and DNA fragmentation was negatively correlated with PLCZ1 expression and the percentage of positively labelled spermatozoa (P < 0.05). The use of equine sperm populations considered to have high versus low PLCZ1 content resulted in significantly higher cleavage rates after ICSI of bovine and equine oocytes, supporting the importance of PLCZ1 for oocyte activation.
Additional keywords: intracytoplasmic sperm injection, PAWP, WBP2NL, DNA fragmentation, oocyte activation.
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