Cryoprotectant role of exopolysaccharide of Pseudomonas sp. ID1 in the vitrification of IVM cow oocytes
Núria Arcarons A , Meritxell Vendrell-Flotats A B , Marc Yeste
C , Elena Mercade D , Manel López-Béjar B and Teresa Mogas A E
+ Author Affiliations
- Author Affiliations
A Department of Animal Medicine and Surgery, Autonomous University of Barcelona, Travessera dels Turons s/n, E-08193, Cerdanyola del Vallès (Barcelona), Spain.
B Department of Animal Health and Anatomy, Autonomous University of Barcelona, Travessera dels Turons s/n, E-08193, Cerdanyola del Vallès (Barcelona), Spain.
C Department of Biology, Institute of Food and Agricultural Technology, University of Girona, Girona, C/ Maria Aurèlia Campany 69, Campus Montilivi, E-17003 Girona, Spain.
D Department de Biology, Health and Environment, Microbiology Section, University of Barcelona, E-08028, Barcelona, Spain.
E Corresponding author. Email: teresa.mogas@uab.cat
Reproduction, Fertility and Development 31(9) 1507-1519 https://doi.org/10.1071/RD18447
Submitted: 28 May 2018 Accepted: 2 April 2019 Published: 16 May 2019
Abstract
Biological molecules isolated from organisms that live under subzero conditions could be used to protect oocytes from cryoinjuries suffered during cryopreservation. This study examined the cryoprotectant role of exopolysaccharides of Pseudomonas sp. ID1 (EPS ID1) in the vitrification of prepubertal and adult cow oocytes. IVM oocytes were vitrified and warmed in media supplemented with 0, 1, 10, 100 or 1000 µg mL−1 EPS ID1. After warming, oocytes were fertilised and embryo development, spindle morphology and the expression of several genes in Day 8 blastocysts were assessed. Vitrification led to significantly lower proportion of prepubertal oocytes exhibiting a normal spindle configuration. In fresh control oocytes and most groups of vitrified adult oocytes, similar percentages of oocytes with a normal spindle configuration were observed. Percentages of Day 8 blastocysts were similar for prepubertal oocytes vitrified in the absence or presence of 1 or 10 µg mL−1 EPS ID1 and for adult oocytes vitrified in the presence of 10 µg mL−1 EPS ID1 compared with non-vitrified oocytes. EPS ID1 supplementation had no effect on solute carrier family 2 member 3 (SLC2A3), ubiquitin-conjugating enzyme E2A (UBE2A) and histone deacetylase 1 (HDAC1) expression in Day 8 blastocysts form adult oocytes. However, supplementation with 10 and 100 µg mL−1 EPS ID1 led to increased expression of genes involved in epigenetic modifications (DNA methyltransferase 3 alpha (DNMT3A) and K (lysine) acetyltransferase 2A (KAT2A)) and apoptosis (BCL2 associated X apoptosis regulator (BAX) and BCL2-like 1 (BCL2L1)). The lowest BAX : BCL2L1 ratio was found in the 10 µg mL−1 EPS ID1-supplemented group. The results suggest that 10 µg mL−1 EPS ID1 added to vitrification and warming media may help protect bovine oocytes against cryodamage.
Additional keywords: calf, chromosomes, cryoprotective agents, embryo development, gene expression regulation, microtubule configuration.
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