Aggregation recovers developmental plasticity in mouse polyploid embryos
Hiroyuki Imai A B , Wataru Fujii C , Ken Takeshi Kusakabe A , Yasuo Kiso A and Kiyoshi Kano
A B D
+ Author Affiliations
- Author Affiliations
A Laboratory of Veterinary Anatomy, Joint-Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 7538515, Japan.
B Laboratory of Developmental Biology, Joint-Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 7538515, Japan.
C Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8517, Japan.
D Corresponding author. Email: kanokiyo@yamaguchi-u.ac.jp
Reproduction, Fertility and Development 31(2) 404-411 https://doi.org/10.1071/RD18093
Submitted: 9 March 2018 Accepted: 21 July 2018 Published: 13 September 2018
Abstract
Tetraploid embryos normally develop into blastocysts and embryonic stem cells can be established from tetraploid blastocysts in mice. Thus, polyploidisation does not seem to be so harmful during preimplantation development. However, the mechanisms by which early mammalian development accepts polyploidisation are poorly understood. In this study, we aimed to elucidate the effect of polyploidisation on early mammalian development and to further comprehend its tolerance using hyperpolyploid embryos produced by repetitive whole genome duplication. We successfully established several types of polyploid embryos (tetraploid, octaploid and hexadecaploid) and studied their developmental potential in vitro. We demonstrated that all types of these polyploid embryos maintained the ability to develop to the blastocyst stage, which implies that mammalian cells might have basic cellular functions in implanted embryos, despite polyploidisation. However, the inner cell mass was absent in hexadecaploid blastocysts. To complement the total number of cells in blastocysts, a fused hexadecaploid embryo was produced by aggregating several hexadecaploid embryos. The results indicated that the fused hexadecaploid embryo finally recovered pluripotent cells in the blastocyst. Thus, our findings suggest that early mammalian embryos may have the tolerance and higher plasticity to adapt to hyperpolyploidisation for blastocyst formation, despite intense alteration of the genome volume.
Additional keywords : aggregated embryo, blastocyst, inner cell mass, polyploidy.
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