CircRNA-9119 regulates the expression of prostaglandin-endoperoxide synthase 2 (PTGS2) by sponging miR-26a in the endometrial epithelial cells of dairy goat
Lei Zhang A , Xiaorui Liu A , Sicheng Che A , Jiuzeng Cui A , Yuexia Liu A , Xiaopeng An A , Binyun Cao A and Yuxuan Song A B
+ Author Affiliations
- Author Affiliations
A College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, P.R. China.
B Corresponding author. Email: yuxuan_song2016@163.com
Reproduction, Fertility and Development 30(12) 1759-1769 https://doi.org/10.1071/RD18074
Submitted: 29 January 2018 Accepted: 7 June 2018 Published: 9 July 2018
Abstract
Circular RNAs (circRNAs) have been found to play important functional roles in epigenetic regulation under certain physiological and pathological conditions. However, knowledge of circRNAs during the development of receptive endometrium (RE) from pre-RE is limited. In the RE of dairy goats, higher circRNA-9119 levels, with lower miR-26a and higher prostaglandin-endoperoxide synthase 2 (PTGS2) levels, were detected. Further study showed that circRNA-9119 decreased levels of miR-26a by acting as a microRNA sponge, and that miR-26a downregulated the expression of PTGS2 via the predicted target site in endometrial epithelial cells (EECs) of dairy goats in vitro. In this way, circRNA-9119 functioned as a competing endogenous RNAs (ceRNA) that sequestered miR-26a, thereby protecting PTGS2 transcripts from miR-26a-mediated suppression in dairy goat EECs in vitro. Furthermore, PTGS2 participated in the regulation of some protein markers for endometrial receptivity in dairy goat EECs in vitro. Thus, a circRNA-9119–miR-26a–PTGS2 pathway in the endometrium was identified, and modulation of circRNA-9119–miR-26a–PTGS2 expression in EECs may emerge as a potential target to regulate the development of RE.
Additional keywords: competing endogenous RNAs (ceRNA), development, embryo implantation, epigenetic regulation, mechanism, non-coding RNAs (ncRNAs), receptive endometrium, reproduction.
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