Fetal bovine serum promotes the development of in vitro porcine blastocysts by activating the Rho-associated kinase signalling pathway
Shimeng Guo A , Shichao Liu A , Gerelchimeg Bou A B , Jia Guo A , Liyuan Jiang A , Zhuang Chai A , Mingming Cai A , Yanshuang Mu A and Zhonghua Liu
A C
+ Author Affiliations
- Author Affiliations
A College of Life Science, Northeast Agricultural University of China, Harbin 150030, China.
B College of Animal Science, Inner Mongolia Agricultural University, Huhhot 018001, China.
C Corresponding author. Email: liu086@126.com
Reproduction, Fertility and Development 31(2) 366-376 https://doi.org/10.1071/RD18070
Submitted: 6 July 2017 Accepted: 13 July 2018 Published: 26 September 2018
Abstract
Fetal bovine serum (FBS) supplementation has beneficial effects on in vitro porcine embryonic development, but the underlying mechanisms are unclear. In the present study we found that the addition of FBS to PZM-3 increased the number of cells in porcine blastocysts and hatching rate in vitro primarily by promoting proliferation of the inner cell mass and further differentiation. Moreover, based on the following results, we surmise that FBS benefits blastocyst development by activating Rho-associated kinase (ROCK) signalling: (1) the ROCK signalling inhibitor Y-27632 decreased the blastocyst rate and the number of cells in blastocysts, whereas FBS rescued the developmental failure induced by Y-27632; (2) the mRNA levels of two ROCK isoforms, ROCK1 and ROCK2, were significantly increased in blastocysts derived from medium containing FBS; and (3) FBS increased RhoA/Rho-kinase expression in the nucleus of embryonic cells. These results indicate that FBS promotes the in vitro development of porcine embryos by activating ROCK signalling in a chemically defined medium.
Additional keywords: blastocyst development, FBS, ROCK signalling pathway.
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