Oestrogen-induced expression of decay accelerating factor is spatiotemporally antagonised by progesterone–progesterone receptor signalling in mouse uterus
Miji Lee A , Hyang Ah Lee B , Mira Park A , Hee Kyoung Park A , Yeon Sun Kim A , Seung Chel Yang A , Hye-Ryun Kim A , Jayeon Kim C D and Haengseok Song A D
+ Author Affiliations
- Author Affiliations
A Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
B Department of Obstetrics and Gynaecology, Kangwon National University School of Medicine, Chuncheon-si, Gangwon-do, 24341, Republic of Korea.
C CHA Fertility Centre Seoul Station, CHA University, Seoul, 04637, Republic of Korea.
D Corresponding authors. Emails: hssong@cha.ac.kr; jayeon_kim@cha.ac.kr
Reproduction, Fertility and Development 30(11) 1532-1540 https://doi.org/10.1071/RD18031
Submitted: 22 January 2018 Accepted: 23 April 2018 Published: 1 June 2018
Abstract
Decay accelerating factor (DAF) is upregulated in the fetoplacental trophoblast, which protects the fetus from maternal complement injury. DAF was found to be downregulated in the endometrium of patients with repeated implantation failure. Thus, we examined the molecular mechanisms of DAF expression regulation by ovarian steroid hormones in the mouse uterus. Immunofluorescence staining demonstrated its exclusive localisation in the apical region of the epithelium in the uterus. Oestrogen (E2) significantly induced Daf mRNA in a time-dependent manner. Progesterone (P4) did not have any significant effect on Daf expression; however, it negatively modulated E2-induced DAF expression and RU486 effectively interfered with the inhibitory action of P4 in the uterus. During early pregnancy DAF was higher on Day 1 of pregnancy, but significantly decreased from Day 3, which is consistent with its E2-dependent regulation. Interestingly, DAF expression seemed to be influenced by the implanting blastocyst on Day 5 and it was gradually increased during preimplantation embryo development with peak levels at blastocyst stages. We demonstrated that E2-dependent DAF expression is antagonised by P4–progesterone receptor signalling in the uterine epithelium. Spatiotemporal regulation of DAF in the uterus and preimplantation embryos suggest that DAF functions as an immune modulator for embryo implantation and early pregnancy in mice.
Additional keywords: complement activation, immune modulation, pregnancy, steroid hormones.
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