Effect of counting chamber depth on the accuracy of lensless microscopy for the assessment of boar sperm motility
Carles Soler A B G , José Á. Picazo-Bueno C , Vicente Micó C , Anthony Valverde A D , Daznia Bompart B , Francisco J. Blasco B , Juan G. Álvarez E F and Almudena García-Molina B
+ Author Affiliations
- Author Affiliations
A University of Valencia, Department of Celular Biology, Functional Biology and Physical Anthropology, Campus Burjassot, C/ Dr Moliner, 50, 46100, Burjassot, Spain.
B Proiser R+D, C/ Catedràtic Agustín Escardino, 9, Building 3 (CUE), Floor 1, 46980, Paterna, Spain.
C University of Valencia, Department of Optics, Campus Burjassot, C/ Dr Moliner, 50, 46100, Burjassot, Spain.
D Technological Institute of Costa Rica, San Carlos Campus, School of Agronomy, 223-21001 Alajuela, Costa Rica.
E Centro ANDROGEN, C/Fernando Macías 8-1°C, 15004, A Coruña, Spain
F Harvard Medical School, Boston, MA, USA.
G Corresponding author. Email: carles.soler@uv.es
Reproduction, Fertility and Development 30(6) 924-934 https://doi.org/10.1071/RD17467
Submitted: 31 October 2017 Accepted: 7 March 2018 Published: 4 May 2018
Journal Compilation © CSIRO 2018 Open Access CC BY-NC-ND
Abstract
Sperm motility is one of the most significant parameters in the prediction of male fertility. Until now, both motility analysis using an optical microscope and computer-aided sperm analysis (CASA-Mot) entailed the use of counting chambers with a depth to 20 µm. Chamber depth significantly affects the intrinsic sperm movement, leading to an artificial motility pattern. For the first time, laser microscopy offers the possibility of avoiding this interference with sperm movement. The aims of the present study were to determine the different motility patterns observed in chambers with depths of 10, 20 and 100 µm using a new holographic approach and to compare the results obtained in the 20-µm chamber with those of the laser and optical CASA-Mot systems. The ISAS®3D-Track results showed that values for curvilinear velocity (VCL), straight line velocity, wobble and beat cross frequency were higher for the 100-µm chambers than for the 10- and 20-µm chambers. Only VCL showed a positive correlation between chambers. In addition, Bayesian analysis confirmed that the kinematic parameters observed with the 100-µm chamber were significantly different to those obtained using chambers with depths of 10 and 20 µm. When an optical analyser CASA-Mot system was used, all kinematic parameters, except VCL, were higher with ISAS®3D-Track, but were not relevant after Bayesian analysis. Finally, almost three different three-dimensional motility patterns were recognised. In conclusion, the use of the ISAS®3D-Track allows for the analysis of the natural three-dimensional pattern of sperm movement.
Additional keywords: CASA-Mot system, kinematic, laser.
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