Haemoglobin expression in in vivo murine preimplantation embryos suggests a role in oxygen-regulated gene expression
M. Lim A B , H. M. Brown
C , K. L. Kind A D , J. Breen A C E , M. R. Anastasi A , L. J. Ritter F , E. K. Tregoweth G , D. T. Dinh A , J. G. Thompson A B and K. R. Dunning A B H
+ Author Affiliations
- Author Affiliations
A Robinson Research Institute, Adelaide Medical School, University of Adelaide, SA 5005, Australia.
B Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, University of Adelaide, Adelaide, SA 5005, Australia.
C South Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia.
D School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, SA 5005, Australia.
E Bioinformatics Hub, University of Adelaide, Adelaide, SA 5005, Australia.
F Adelaide Medical School, University of Adelaide, Adelaide, SA 5005, Australia.
G Sansom Institute for Health Research, University of South Australia, Adelaide, SA 5005, Australia.
H Corresponding author. Email: kylie.dunning@adelaide.edu.au
Reproduction, Fertility and Development 31(4) 724-734 https://doi.org/10.1071/RD17321
Submitted: 7 August 2017 Accepted: 24 October 2018 Published: 28 November 2018
Abstract
Haemoglobin expression is not restricted to erythroid cells. We investigated the gene expression of the haemoglobin subunits haemoglobin, alpha adult chain 1 (Hba-a1) and haemoglobin, beta (Hbb), 2,3-bisphosphoglycerate mutase (Bpgm) and the oxygen-regulated genes BCL2/adenovirus E1B interacting protein 3 (Bnip3), solute carrier family 2 (facilitated glucose transporter), member 1 (Slc2a1) and N-myc downstream regulated gene 1 (Ndrg1) in the murine preimplantation embryo, comparing in vivo to in vitro gene expression. Relatively high levels of Hba-a1 and Hbb were expressed in vivo from the 2-cell to blastocyst stage; in contrast, little or no expression occurred in vitro. We hypothesised that the presence of haemoglobin in vivo creates a low oxygen environment to induce oxygen-regulated gene expression, supported by high expression of Slc2a1 and Ndrg1 in in vivo relative to in vitro embryos. In addition, analysis of an in vitro-derived human embryo gene expression public dataset revealed low expression of haemoglobin subunit alpha (HBA) and HBB, and high expression of BPGM. To explore whether there was a developmental stage-specific effect of haemoglobin, we added exogenous haemoglobin either up to the 4-cell stage or throughout development to the blastocyst stage, but observed no difference in blastocyst rate or the inner cell mass to trophectoderm cell ratio. We conclude that haemoglobin in the in vivo preimplantation embryo raises an interesting premise of potential mechanisms for oxygen regulation, which may influence oxygen-regulated gene expression.
Additional keywords: gene regulation, IVF.
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