Lipid profile of bovine blastocysts exposed to insulin during in vitro oocyte maturation
Denise Laskowski A E F , Göran Andersson B E , Patrice Humblot A E , Marc-André Sirard C , Ylva Sjunnesson A E , Christina R. Ferreira D , Valentina Pirro D and Renée Båge A E
+ Author Affiliations
- Author Affiliations
A Department of Clinical Sciences, Swedish University of Agricultural Sciences, PO Box 7054, SE-750 07 Uppsala, Sweden.
B Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, PO Box 7023, SE-750 07 Uppsala, Sweden.
C Departement des Sciences Animales, Centre de Recherche en Biologie de la Reproduction, Pavillon Des Services, Local 2732, University Laval, Québec G1V 0A6, Canada.
D Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907-2084, USA.
E Centre for Reproductive Biology in Uppsala (CRU), Box 7054, 75007 Uppsala, Sweden.
F Corresponding author. Email: denise.laskowski@slu.se
Reproduction, Fertility and Development 30(9) 1253-1266 https://doi.org/10.1071/RD17248
Submitted: 20 February 2017 Accepted: 1 March 2018 Published: 16 April 2018
Abstract
Insulin is a key hormone with important functions in energy metabolism and is involved in the regulation of reproduction. Hyperinsulinaemia is known to impair fertility (for example, in obese mothers); therefore, we aimed to investigate the impact of elevated insulin concentrations during the sensitive period of oocyte maturation on gene expression and lipid profiles of the bovine Day-8 embryo. Two different insulin concentrations were used during in vitro oocyte maturation (INS10 = 10 µg mL−1 and INS0.1 = 0.1 µg mL−1) in order to observe possible dose-dependent effects or thresholds for hyperinsulinaemia in vitro. By investigating gene expression patterns by an mRNA microarray in combination with lipid profile analysis by desorption electrospray ionisation-mass spectrometry (DESI-MS) of embryos derived from insulin-treated oocytes, we gained further insights regarding molecular responses of embryos to insulin provocation during the first days of development. Lipid metabolism appeared to be influenced on multiple levels according to gene expression results but the profiles collected in positive-ion mode by DESI-MS (showing mostly ubiquinone, cholesteryl esters and triacylglycerols) did not differ significantly from controls. There are parallels in follicular development of ruminants and humans that make this bovine model relevant for comparative research on early human embryonic development during hyperinsulinaemia.
Additional keywords: early embryonic development, hyperinsulinaemia, lipid metabolism, metabolic imbalance.
References
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