Glutamine protects rabbit spermatozoa against oxidative stress via glutathione synthesis during cryopreservation
Zhendong Zhu A , Xiaoteng Fan A , Yinghua Lv A , Yan Lin B , De Wu B and Wenxian Zeng A C
+ Author Affiliations
- Author Affiliations
A College of Animal Science and Technology, Northwest A&F University, 22 Xi-nong Road, Yangling, Shaanxi, 712100, China.
B Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, 211 Hui-min Road, Wenjiang, Chengdu, Sichuan, 611100, China.
C Corresponding author. Email: zengwenxian2013@126.com
Reproduction, Fertility and Development 29(11) 2183-2194 https://doi.org/10.1071/RD17020
Submitted: 25 October 2016 Accepted: 7 February 2017 Published: 27 March 2017
Abstract
Mammalian spermatozoa are extremely susceptible to high doses of reactive oxygen species (ROS). The aim of the present study was to investigate the potential role of glutamine in protecting rabbit spermatozoa against ROS stress during cryopreservation and post-thaw incubation. Freshly ejaculated semen was diluted with Tris–citrate–glucose extender supplemented with glutamine. The addition of 20 mM glutamine significantly improved sperm motility, acrosome integrity, membrane integrity and mitochondrial activity. Meanwhile, 20 mM glutamine addition decreased lipid peroxidation and DNA damage in frozen–thawed spermatozoa. Interestingly, supplementation with 20 mM glutamine led to increases in glutathione content and γ-glutamyl cysteine synthetase and glutathione peroxidase activity, with concomitant decreases in ROS levels during cryopreservation and post-thaw incubation. In conclusion, the addition of glutamine to extender solutions protects rabbit spermatozoa from ROS attack by enhancing glutathione synthesis.
Additional keywords: reactive oxygen species, semen.
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