Phyto-oestrogens affect fertilisation and embryo development in vitro in sheep
Anna Aryani Amir A B C , Jennifer M. Kelly D , David O. Kleemann D , Zoey Durmic A B , Dominique Blache A B and Graeme B. Martin A B
+ Author Affiliations
- Author Affiliations
A School of Agriculture and Environment, The University of Western Australia, Crawley, WA 6009, Australia.
B UWA Institute of Agriculture, The University of Western Australia, Crawley, WA 6009, Australia.
C Institut Pertanian Tropika dan Sekuriti Makanan, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia.
D South Australian Research and Development Institute, Turretfield Research Centre, Rosedale, SA 5350, Australia.
E Corresponding author. Email: graeme.martin@uwa.edu.au
Reproduction, Fertility and Development 30(8) 1109-1115 https://doi.org/10.1071/RD16481
Submitted: 29 November 2016 Accepted: 22 December 2017 Published: 16 February 2018
Abstract
Phyto-oestrogens such as isoflavones are natural compounds that can profoundly affect reproductive function. In the present study, we tested whether including isoflavone compounds (genistein, biochanin A, formononetin) in the maturation medium would affect the outcomes for ovine oocytes in vitro. Each isoflavone compound was evaluated at five concentrations (0, 2.5, 5, 10, 25 µg mL−1) and the entire protocol was repeated four times. Cumulus–oocyte complexes were randomly allocated to the treatments, then fertilised and cultured in vitro. Compared with control (0 µg mL−1), the lower concentrations of isoflavone (2.5, 5 and 10 µg mL−1) had no detectable effect on the rates of cleavage or embryo development, or on embryo total cell counts (TCC). However, the highest concentration (25 µg mL−1) of all three isoflavones exerted a variety of effects (P < 0.05): genistein decreased cleavage rate, blastocyst rate and blastocyst efficiency (blastocysts produced per 100 oocytes); biochanin A decreased cleavage rate and blastocyst efficiency; and formononetin decreased blastocyst rate and blastocyst efficiency. Biochanin A (25 µg mL−1) reduced embryo TCC specifically at the hatched blastocyst stage (P < 0.05). We conclude that the presence of isoflavones at 25 µg mL−1 during IVM decreases the cleavage rate and inhibits blastocyst hatching.
Additional keywords: biochanin A, blastocyst, formononetin, genistein, oocytes.
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