Effect of spermatozoa motility hyperactivation factors and gamete coincubation duration on in vitro bovine embryo development using flow cytometrically sorted spermatozoa
Luis B. Ferré A F , Yanina Bogliotti B , James L. Chitwood B , Cristóbal Fresno C , Hugo H. Ortega D , Michael E. Kjelland E and Pablo J. Ross B F
+ Author Affiliations
- Author Affiliations
A Instituto Nacional de Tecnología Agropecuaria (INTA), Ruta Nacional 34, Km 227, Rafaela (S2300ICB), Santa Fe, Argentina.
B Department of Animal Science, One Shields Avenue, University of California, Davis, CA 95616, USA.
C Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Facultad de Ingeniería, Universidad Católica de Córdoba, Av. Armada Argentina 3555, Córdoba (X5016DHK), Argentina.
D Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral/CONICET, R.P. Kreder 2805, Esperanza (S3080HOF), Santa Fe, Argentina.
E Conservation, Genetics and Biotech, LLC, P.O. Box 821522, Vicksburg, MS 39182, USA.
F Corresponding authors. Emails: ferre.luis@inta.gob.ar; pross@ucdavis.edu
Reproduction, Fertility and Development 29(4) 805-814 https://doi.org/10.1071/RD15289
Submitted: 17 July 2015 Accepted: 1 December 2015 Published: 24 February 2016
Abstract
The aim of the present study was to evaluate the effects of sperm motility enhancers and different IVF times on cleavage, polyspermy, blastocyst formation, embryo quality and hatching ability. In Experiment 1, sex-sorted X chromosome-bearing Bos taurus spermatozoa were incubated for 30 min before 18 h fertilisation with hyperactivating factors, namely 10 mM caffeine (CA), 5 mM theophylline (TH), 10 mM caffeine and 5 mM theophylline (CA + TH); and untreated spermatozoa (control). In Experiment 2, matured B. taurus oocytes were fertilised using a short (8 h) or standard (18 h) fertilisation length, comparing two different fertilisation media, namely synthetic oviducal fluid (SOF) fertilisation medium (SOF-FERT) and M199 fertilisation medium (M199-FERT). Cleavage and blastocyst formation rates were significantly higher in the CA + TH group (77% and 27%, respectively) compared with the control group (71% and 21%, respectively). Cleavage rates and blastocyst formation were significantly lower for the shortest fertilisation time (8 h) in M199-FERT medium (42% and 12%, respectively). The SOF-FERT medium with an 8 h fertilisation time resulted in the highest cleavage rates and blastocyst formation (74% and 29%, respectively). The SOF-FERT medium produced the highest embryo quality (50% Grade 1) and hatching rate (66%). Motility enhancers did not affect polyspermy rates, whereas polyspermy was affected when fertilisation length was extended from 8 h (3%) to 18 h (9%) and in M199-FERT (14%) compared with SOF-FERT (6%). We conclude that adding the motility enhancers CA and TH to sex sorted spermatozoa and Tyrode’s albumin lactate pyruvate (TALP)-Sperm can improve cleavage and embryo development rates without increasing polyspermy. In addition, shortening the oocyte–sperm coincubation time (8 h) resulted in similar overall embryo performance rates compared with the prolonged (18 h) interval.
Additional keywords: IVF, sexed spermatozoa.
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