Evidence that fibroblast growth factor 10 plays a role in follicle selection in cattle
A. C. S. Castilho A E , C. A. Price B , F. Dalanezi A , R. L. Ereno A , M. F. Machado A , C. M. Barros A , B. G. Gasperin C , P. B. D. Gonçalves C and J. Buratini D
+ Author Affiliations
- Author Affiliations
A Departamento de Farmacologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, Distrito de Rubião Junior, Botucatu, São Paulo 18618-970, Brasil.
B Centre de recherche en reproduction animale, Faculté de médecine vétérinaire, Université de Montréal, 3200 Sicotte, Saint-Hyacinthe, Québec J2S 7C6, Canada.
C Laboratório de Biotecnologia e Reprodução Animal, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, Rio Grande do Sul 97105-900, Brasil.
D Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, Botucatu, São Paulo 18618-970, Brasil.
E Corresponding author. Email: buratini@ibb.unesp.br
Reproduction, Fertility and Development 29(2) 234-243 https://doi.org/10.1071/RD15017
Submitted: 14 January 2015 Accepted: 16 June 2015 Published: 21 July 2015
Abstract
There is evidence that regulation of follicle selection in cattle involves locally produced growth factors. In the present study, we investigated the expression of members of the fibroblast growth factor (FGF) 7 family during follicle deviation. The largest and second largest follicles were recovered during the second day of a synchronised follicle wave and the future dominant and future subordinate follicles were identified based on diameter and cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1) mRNA levels in granulosa cells. Theca cells of the future dominant follicle contained less mRNA encoding FGF7 and FGF10 compared with those from the future subordinate follicle 2.5 days after ovulation, before a significant difference between the diameters of the future dominant and future subordinate follicles could be observed, but FGF22 mRNA levels did not change. Levels of mRNA encoding FGF receptors FGFR1B and FGFR2B in theca and granulosa cells, respectively, were lower in the future dominant follicle compared with the future subordinate follicle. Addition of FGF10 to granulosa cells in vitro significantly decreased oestradiol secretion, as well as CYP19A1, FSH receptor (FSHR) and insulin-like growth factor 1 receptor (IGF1R) mRNA abundance, whereas FGF22 had no effect. We conclude that FGF10 and FGFR2B expression is increased in the future subordinate follicle before morphological deviation, which may contribute to follicle selection.
Additional keywords: antral follicles, bovine, deviation, ovary.
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