Food deprivation and social inequality may lead to oxidative damage: a study on the preventive role of melatonin in the male rat reproductive system
Shiva Nasiraei-Moghadam A E , Kazem Parivar A , Abolhasan Ahmadiani B , Mansoureh Movahhedin C and Mohammad-Reza Vaez Mahdavi D
+ Author Affiliations
- Author Affiliations
A Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran.
B Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
C Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
D Department of Physiology, Faculty of Medicine, Shahed Medical University, Tehran, Iran.
E Corresponding author. Email: shiva_nasiraei@yahoo.com
Reproduction, Fertility and Development 28(8) 1232-1239 https://doi.org/10.1071/RD14432
Submitted: 6 September 2014 Accepted: 13 December 2014 Published: 16 February 2015
Abstract
Spermatogenic cells are susceptible to oxidative stress and apoptosis. Food deprivation (FD) has been reported as a stressor that could increase reactive oxygen species. In the present study, FD-induced oxidative stress and apoptosis, as well as the protective effects of melatonin, were evaluated in the testes. Wistar rats in the control group were fed a standard diet, whereas a sham group was administered saline as the melatonin vehicle. A third group received daily injections of melatonin (5 mg kg–1 bodyweight). These rats were further divided into four groups of rats that were either subjected to FD, FD + isolation, FD + melatonin injection and FD + melatonin injection + isolation. Testicular tissues were evaluated for malondialdehyde (MDA) and reduced glutathione (GSH) concentrations, as well as and DNA damage. FD increased MDA and reduced GSH concentrations, whereas melatonin treatment improved these parameters. Immunohistochemistry for capsase-3 and terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling revealed that the number of apoptotic cells was increased in rats subjected to FD alone. Melatonin treatment offset the number of apoptotic cells following FD. The results provide evidence that FD can increase oxidative stress, leading to activation of apoptosis, and that melatonin has the ability to protect the testes against oxidative damage induced by FD.
Additional keywords: apoptosis, testis.
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