Involvement of prolactin in the meloxicam-dependent inflammatory response of the gonadotropic axis to prolonged lipopolysaccharide treatment in anoestrous ewes
Andrzej P. Herman A C , Agata Krawczyńska A , Joanna Bochenek A , Hanna Antushevich A , Anna Herman B and Dorota Tomaszewska-Zaremba AA Polish Academy of Sciences, The Kielanowski Institute of Animal Physiology and Nutrition, 05-110 Jabłonna, Poland.
B The Academy of Cosmetics and Health Care, 13 Podwale Street, 00-252 Warsaw, Poland.
C Corresponding author. Email: andrewherman@wp.pl
Reproduction, Fertility and Development 28(7) 914-923 https://doi.org/10.1071/RD13435
Submitted: 21 December 2013 Accepted: 20 October 2014 Published: 28 November 2014
Abstract
An immune challenge can affect the reproductive process in females. Peripheral administration of bacterial endotoxin (lipopolysaccharide; LPS) decreases LH secretion and disrupts ovarian cyclicity. The aim of the present study was to determine the effects of a cyclo-oxygenase (COX)-2 inhibitor (meloxicam) on gonadotropin-releasing hormone (GnRH) and LH secretion in anoestrous ewes during systemic inflammation induced by LPS. LPS (400 ng kg–1 per day) suppressed LH release. In three individuals, meloxicam (500 μg kg–1, i.v.) abolished LPS-induced LH suppression. In another three ewes LH was ineffective. Similar changes were observed in hypothalamic GnRH expression. The effect of meloxicam depended on the circulating level of prolactin: meloxicam abolished inflammatory-dependent suppression of GnRH and LH secretion when plasma prolactin levels were similar to those in untreated animals, but was ineffective in those with elevated levels of prolactin. We conclude that COX-2 inhibitors minimise the negative effect of inflammation on the reproductive system but that this effect may be antagonised by prolactin.
Additional keywords: gonadotrophin-releasing hormone, hypothalamus, LH.
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