Cryotolerance and global gene-expression patterns of Bos taurus indicus and Bos taurus taurus in vitro- and in vivo-produced blastocysts
Mateus J. Sudano A F , Ester S. Caixeta B , Daniela M. Paschoal A , Alicio Martins
+ Author Affiliations
- Author Affiliations
A São Paulo State University, School of Veterinary Medicine and Animal Science, Department of Animal Reproduction and Veterinary Radiology, Rubião Jr. s/n°, 18618-970, Botucatu–SP, Brazil.
B University of José Rosário Vellano (UNIFENAS), Department of Animal Reproduction, 37130-000, Alfenas-MG, Brazil.
C São Paulo State University, School of Veterinary Medicine, Department of Clinical Surgery and Animal Reproduction, 16050-680, Araçatuba-SP, Brazil.
D Embrapa Southeast Cattle, PO Box 339, 13560-970, São Carlos-SP, Brazil.
E São Paulo State University, Department of Physiology, Rubião Jr. s/n°, 18618-970, Botucatu–SP, Brazil.
F Corresponding authors. Email: mjsudano@gmail.com; fernanda@fmvz.unesp.br
Reproduction, Fertility and Development 26(8) 1129-1141 https://doi.org/10.1071/RD13099
Submitted: 22 March 2013 Accepted: 9 August 2013 Published: 12 September 2013
Abstract
In a 2 × 2 factorial experimental design, embryo development, cryotolerance and global gene expression of Nellore (Bos taurus indicus) and Simmental (Bos taurus taurus) blastocysts produced in vitro (IVP) and in vivo (multiple ovulation derived embryo, MODE) were assessed. Blastocyst production was higher in Nellore than in Simmental (47.7 ± 2.0% vs 27.0 ± 2.0%) cows. The total numbers of ova or embryos recovered (5.5 ± 0.9 vs 3.7 ± 0.8) and transferable embryos (3.8 ± 1.0 vs 2.3 ± 0.8) per cow were not different between breeds. Simmental and MODE (34.6% and 38.5%, n = 75 and 70) blastocysts had higher survival rates after cryopreservation compared with Nellore and IVP (20.2% and 18.1%, n = 89 and 94) embryos, respectively. Differences between transcriptomes were addressed by principal-component analysis, which indicated that gene expression was affected by subspecies (158 genes), origin (532 genes) and interaction between both subspecies and origin (53 genes). Several functional processes and pathways relevant to lipid metabolism and embryo viability involving differentially expressed genes were identified. The lipid metabolism-related genes were upregulated in Simmental (AUH and ELOVL6) and IVP (ACSL3 and ACSL6) blastocysts. The expression profiles of genes related to mitochondrial metabolism (ATP5B), oxidative stress (GPX4), apoptosis (DAD1, DAP, PRDX2), heat shock (HSPA5), pregnancy (IFNT2, PAG2) and cell differentiation (KRT18) varied between experimental groups.
Additional keywords: bovine, cryopreservation, cryosurvival, transcriptome.
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