ART do not increase the risk of Y-chromosome microdeletion in 19 candidate genes at AZF regions
Xiao-Hong Liu A B C D , Li-Ying Yan A B C , Cui-Ling Lu A B C , Rong Li A B C , Xiao-Hui Zhu A B C , Hong-Yan Jin A B C , Yan Zhang A B C , Wen-Xin Zhang A B C , Su-Hong Gao D and Jie Qiao A B C E
+ Author Affiliations
- Author Affiliations
A Center for Reproductive Medicine, Department of Obstetrics and Gynaecology, Peking University Third Hospital, No. 49, Huayuan North Road, Haidian District, Beijing 100191, China.
B Key Laboratory of Assisted Reproduction, Ministry of Education, No. 49, Huayuan North Road, Haidian District, Beijing 100191, China.
C Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, No. 49, Huayuan North Road, Haidian District, Beijing 100191, China.
D Beijing Haidian Maternal and Child Health Hospital, No.33, Haidian South Road, Haidian District, Beijing 100080, China.
E Corresponding author. Email: jie.qiao@263.net
Reproduction, Fertility and Development 26(6) 778-786 https://doi.org/10.1071/RD13092
Submitted: 19 March 2013 Accepted: 11 May 2013 Published: 11 June 2013
Abstract
Y-chromosome microdeletions (YCMs) have been found at a much higher rate in infertile men than fertile controls. A specific deletion in the azoospermia factor locus (AZF) at Yq11 is significantly associated with male infertility. Whether assisted reproductive technology (ART) increases the risk of YCM in ART-derived offspring remains unclear. In this study the occurrence of YCM in 199 fathers and their 228 sons (Chinese, Han ethnicity), including 85 offspring conceived by IVF, 73 by intra-cytoplasmic sperm injection (ICSI) and 70 by natural conception, was investigated. Nineteen candidate genes related to YCM were analysed by multiplex ligation-dependent probe amplification. We identified one de novo YCM from 70 naturally-conceived offspring and none from 158 ART-conceived offspring and found no statistical significance between these two groups. There was no statistically-significant difference in the detection rate of the father’s Y-chromosome microdeletion group: IVF 10.7% (8/75), ICSI 3.2% (2/63), natural conception 8.2% (5/61). These results suggest that ART does not increase the risk of YCM in male offspring.
Additional keywords: assisted reproductive technology, ICSI, male infertility, outcomes.
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