Zona pellucida birefringence and meiotic spindle visualisation of human oocytes are not influenced by IVM technology
Marjan Omidi A , Mohammad Ali Khalili A B C , Sareh Ashourzadeh A and Marzieh Rahimipour A
+ Author Affiliations
- Author Affiliations
A Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd 8247679433, Iran.
B Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd 8247679433, Iran.
C Corresponding author. Email: khalili59@hotmail.com
Reproduction, Fertility and Development 26(3) 407-413 https://doi.org/10.1071/RD13001
Submitted: 2 January 2013 Accepted: 18 February 2013 Published: 2 April 2013
Abstract
The aim of the present study was to investigate the relationship between the presence of the meiotic spindle and zona pellucida (ZP) birefringence with morphology of in vivo- and in vitro-matured human oocytes. Germinal vesicles (n = 47) and MI (n = 38) oocytes obtained from stimulated ovaries of patients undergoing intracytoplasmic sperm injection (ICSI) underwent IVM. Using a PolScope (OCTAX PolarAID; Octax, Herbon, Germany), the presence of spindles and ZP birefringence was assessed in both in vivo-matured (n = 56) and IVM (n = 56) oocytes. In addition, the morphology of each matured oocyte was evaluated microscopically. There were insignificant differences for ZP birefringence and meiotic spindle between the in vivo-matured and IVM MII oocytes. Subanalysis revealed that the rates of morphologically abnormal oocytes did not differ significantly between the two groups, except in the case of irregular shape (P = 0.001), refractile body (P = 0.001) and fragmented polar body (P = 0.03), which were higher in IVM oocytes. In the case of in vivo-matured oocytes, a significantly higher percentage of oocytes with intracytoplasmic and both intra- and extracytoplasmic abnormalities have a low birefringent ZP (P = 0.007 and P = 0.02, respectively). There was no relationship between morphological abnormalities and spindle detection. The findings suggest that clinical IVM is a safe technology that maintains the high maturation rate and integrity of oocytes. In addition, the use of the non-invasive PolScope is recommended for the detection of oocytes most suitable for ICSI.
Additional keywords: oocyte morphology, PolScope.
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