Immunolocalisation and oestrogen regulation of small proline-rich protein 2a protein in the mouse uterus
Hyang-Ah Lee A H , Hye-Ryun Kim B H , Young Jin Lee C , Seung-Joon Lee D , Woo Jin Kim D , Seon-Sook Han D , Se-Ran Yang E G , Heung-Myong Woo F G , Sunghun Na A , Haengseok Song B I and Seok-Ho Hong D G I
+ Author Affiliations
- Author Affiliations
A Department of Obstertics and Gynecology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
B Department of Biomedical Science, CHA University, Seoul 135-081, South Korea.
C Center for Biomedical Engineering and Technology and Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
D Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
E Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
F College of Veterinary Medicine, Kangwon National Univeristy, Chuncheon 200-701, South Korea.
G Stem Cell Institute, Kangwon National University, Chuncheon 200-701, South Korea.
H These authors equally contributed to this work.
I Corresponding authors. Emails: hssong@cha.ac.kr; shhong@kangwon.ac.kr
Reproduction, Fertility and Development 26(5) 682-689 https://doi.org/10.1071/RD12408
Submitted: 24 December 2012 Accepted: 21 April 2013 Published: 5 June 2013
Abstract
Small proline-rich protein 2a (Sprr2a) is one of the structural components of the cornified keratinocyte cell envelope that contributes to form a protective barrier in the skin against dehydration and environmental stress. Interestingly, Sprr2a mRNA is detected in the mouse uterus and is regulated by 17β-oestradiol (E2). In the present study, we investigated the effects of E2 and oestrogenic compounds on the regulation and localisation of Sprr2a protein in the mouse uterus. Immunohistochemical staining revealed that Sprr2a protein is detected only in the adult uterus, and not in the ovary, oviduct or testis. We also demonstrated that Sprr2a protein is tightly regulated by E2 in the mouse uterus and exclusively detected in luminal and glandular epithelial cells. Furthermore, Sprr2a is dose-dependently induced by oestrogenic compounds such as bisphenol A and 4-tert-octylphenol. Collectively, our studies suggest that Sprr2a protein may have a unique function in physiological events in the mouse uterus and can be used as an indicator to detect compounds with oestrogenic activity in the mouse uterus.
Additional keywords: cornified envelope, oestrogenic compound, oestrous.
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