Actions of oestradiol and progesterone on the prostate in female gerbils: reversal of the histological effects of castration
Marianna Zanatelli A , Diego A. L. Silva A , Filipe Z. Shinohara A , Rejane M. Góes C , Fernanda C. A. Santos B , Patricia S. L. Vilamaior C and Sebastião R. Taboga C D
+ Author Affiliations
- Author Affiliations
A Department of Cell Biology, Institute of Biology, State University of Campinas, R. Monteiro Lobato 255, 13083-970, Campinas, São Paulo, Brazil.
B Department of Morphology, Institute of Biological Sciences, Federal University of Goiás, CP 131, 74001-970, Goiânia, Goiás, Brazil.
C Laboratory of Microscopy and Microanalysis, Department of Biology, São Paulo State University, R Cristovão Colombo, 2265, 15054-000, São José do Rio Preto, São Paulo, Brazil.
D Corresponding author. Email: taboga@ibilce.unesp.br
Reproduction, Fertility and Development 26(4) 540-550 https://doi.org/10.1071/RD12302
Submitted: 18 September 2012 Accepted: 18 March 2013 Published: 16 May 2013
Abstract
The female prostate is a functionally active gland in several mammalian species, including humans and rodents. Investigations of prostate morphophysiology during the phases of the oestrous cycle have shown that the female prostate is influenced by fluctuations in serum concentrations of oestradiol (E2) and progesterone (P4). The aim of the present study was to evaluate the effect of combined prolonged administration of E2 and P4 on the prostate in ovariectomised female gerbils. Ovariectomy caused atrophy and decreased glandular secretory activity. Administration of E2 and P4 (0.1 mg kg–1 diluted in 0.1 mL of mineral oil, every 48 h over 30 days) resulted in a recovery of overall prostate structure, as evidenced by increased epithelial height, mass and prostatic secretory activity, without leading the appearance of significant lesions. Evaluation of androgen receptor (AR) expression revealed increased immunoreactivity in the E2+P4-treated group. Immunostaining for oestrogen receptor (ER) α was decreased in the castrated groups, but increased in the group subjected to hormone treatment. There were no significant differences in ERβ immunoreactivity among the groups. Assessment of cell proliferation revealed greater immunoreactivity in the treated group. Together, the results indicate that the interaction between E2 and P4 may be responsible for maintaining female prostate gland histophysiology.
Additional keyword: ovariectomy.
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