Claudin-8 expression in Sertoli cells and putative spermatogonial stem cells in the bovine testis
Mary McMillan A B D , Nicholas Andronicos B , Rhonda Davey A B , Sally Stockwell A C , Geoff Hinch D and Sabine Schmoelzl A B D E
+ Author Affiliations
- Author Affiliations
A CSIRO Food Futures National Research Flagship, North Ryde, NSW 2113, Australia.
B CSIRO Animal, Food and Health Sciences, F. D. McMaster Laboratory, Armidale, NSW 2350, Australia.
C CSIRO Animal, Food and Health Sciences, Queensland Bioscience Precinct, St Lucia, Qld 4067, Australia.
D School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia.
E Corresponding author. Email: sabine.schmoelzl@csiro.au
Reproduction, Fertility and Development 26(5) 633-644 https://doi.org/10.1071/RD12259
Submitted: 8 August 2012 Accepted: 16 April 2013 Published: 15 May 2013
Abstract
Adhesion molecules are expressed by both adult and embryonic stem cells, with different classes of adhesion molecules involved in cell-membrane and intercellular contacts. In this study the expression of the adhesion molecule claudin-8 (CLDN8), a tight-junction protein, was investigated as a potential marker for undifferentiated spermatogonia in the bovine testis. We found that CLDN8 was expressed by both spermatogonia and a subset of Sertoli cells in the bovine testis. We also showed co-expression of GFRα1 in testis cells with CLDN8 and with Dolichos biflorus agglutinin–fluorescein isothiocyanate (DBA–FITC) staining. We observed co-enrichment of spermatogonia and CLDN8-expressing Sertoli cells in DBA–FITC-assisted magnetic-activated cell sorting (MACS), an observation supported by results from fluorescence-activated cell sorting analysis, which showed CLDN8-expressing cells were over-represented in the MACS-positive cell fraction, leading to the hypothesis that CLDN8 may play a role in the spermatogonial stem-cell niche.
Additional keywords: adhesion molecule, stem-cell niche, tight junction.
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