Effect of monosaccharide sugars on LH-induced differentiation and sugar transport facilitator (SLC2A) expression in sheep theca cells in vitro
B. K. Campbell A D , N. R. Kendall A C , V. Onions A , L. Guo A and R. J. Scaramuzzi B
+ Author Affiliations
- Author Affiliations
A Division of Human Development, School of Clinical Sciences, University of Nottingham, Queen’s Medical Centre, Nottingham NG7 2UH, UK.
B Department of Veterinary Basic Sciences, The Royal Veterinary College, Hawkshead Lane, Herts AL9 7TA, UK.
C Present address: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK.
D Corresponding author. Email: bruce.campbell@nottingham.ac.uk
Reproduction, Fertility and Development 26(3) 453-461 https://doi.org/10.1071/RD12064
Submitted: 29 February 2012 Accepted: 26 February 2013 Published: 28 May 2013
Abstract
The aim of the present study was to investigate the effects of glucose, galactose and fructose on the LH-induced differentiation and mRNA expression of sugar transport facilitators (SLC2A) by sheep thecal cells derived from small antral follicles cultured under serum-free conditions for 6 days. The dose and type of monosaccharide had a significant effect on LH-induced androstenedione production by theca cells and there was a significant interaction (P < 0.001). Glucose and galactose were used with equal efficiency so that cell numbers and androstenedione production at the end of the culture were comparable. Pharmacological doses of glucose (16.7 mM) inhibited steroidogenesis (P < 0.05). Cell numbers and androstenedione production by cells cultured with fructose were lower than for cells cultured with either glucose or galactose (P < 0.001). None of the monosaccharides resulted in the production of lactate. Expression of SLC2A1, SLC2A4 and SLC2A8, but not SLC2A5, mRNA was detected in fresh and cultured theca cells. Large doses (16.7 mM) of glucose and fructose, but not galactose, suppressed (P < 0.05) SLC2A expression. The results show that glucose and galactose, but not fructose, are readily metabolised via oxidative pathways to support LH-induced differentiation of sheep theca cells. Further work is required to determine the mechanisms resulting in these differences in relation to the established effects of nutrition on reproductive function.
Additional keywords: fructose, galactose, glucose.
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