The effects of n-acetyl-l-cysteine supplementation on in vitro porcine oocyte maturation and subsequent fertilisation and embryonic development
B. D. Whitaker A B C , S. J. Casey A and R. Taupier A
+ Author Affiliations
- Author Affiliations
A Department of Agriculture, Ferrum College, PO Box 1000, Ferrum, VA 24088, USA.
B Present address: Department of Animal Science, The University of Findlay, 1000 North Main Street, Findlay, OH 45840, USA.
C Corresponding author. Email: whitaker@findlay.edu
Reproduction, Fertility and Development 24(8) 1048-1054 https://doi.org/10.1071/RD12002
Submitted: 21 June 2011 Accepted: 7 March 2012 Published: 10 April 2012
Abstract
The effects of supplementation with 1.5 mM n-acetyl-l-cysteine (NAC) during in vitro oocyte maturation were studied. Oocytes were supplemented with 1.5 mM NAC during maturation for 0 to 24 h, 24 to 48 h, or 0 to 48 h then subjected to IVF and embryo development. Oocytes were evaluated after maturation for intracellular glutathione concentration, superoxide dismutase and glutathione peroxidase activities and DNA fragmentation. Fertilisation and embryonic development success were also evaluated. There was no effect of treatment on intracellular glutathione concentrations, enzyme activities or fertilisation success rates. Supplementing NAC during maturation significantly decreased (P < 0.05) the percentage of oocytes with fragmented DNA compared with no NAC supplementation. Supplementing NAC from 24 to 48 h or 0 to 48 h resulted in a significantly higher (P < 0.05) percentage of oocytes with male pronuclei than for oocytes from the other treatment groups. There was no difference in the percentage of embryos cleaved by 48 h after IVF between treatment groups. Supplementing NAC from 24 to 48 h or 0 to 48 h resulted in a significantly higher (P < 0.05) percentage of embryos reaching the blastocyst stage by 144 h after IVF compared with the other treatment groups. These results indicate that supplementation of the oocyte maturation medium with 1.5 mM NAC, specifically during the last 24 h, improves male pronucleus formation and blastocyst development in pigs.
Additional keywords: antioxidants, in vitro fertilisation, oxidative stress, pigs, polyspermy.
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