Employing mated females as recipients for transfer of cloned dog embryos
Geon A Kim A , Hyun Ju Oh A , Jung Eun Park A , Min Jung Kim A , Eun Jung Park A , Sang Hyun Lim A B , Sung Keun Kang B , Goo Jang A and Byeong Chun Lee A C
+ Author Affiliations
- Author Affiliations
A Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, 151-744, Korea.
B Central Research Center, RNL BIO, Seoul, 153-768, Korea.
C Corresponding author. Email: bclee@snu.ac.kr
Reproduction, Fertility and Development 25(4) 700-706 https://doi.org/10.1071/RD11221
Submitted: 31 August 2011 Accepted: 29 May 2012 Published: 17 July 2012
Abstract
It has been suggested that co-transferring parthenogenetic embryos could improve the pregnancy success rate with cloned embryos in mammals. As an alternative to co-transferring parthenotes, in dogs we employed recipient females that possessed in vivo-fertilised embryos as a result of mating to determine whether mated bitches could be suitable recipients for cloned embryos. The effect of using mated recipients on implantation and pregnancy rates of canine somatic cell nuclear transfer embryos was also determined. Cloned embryos were transferred into the oviducts of naturally synchronous females that had mated with male dogs before ovulation. The pregnancy rate appeared to be similar between mated recipients (50%) and non-mated recipients (28.57%; P > 0.05). However, the delivery rate of cloned pups was significantly higher in mated recipients than non-mated recipients (10.53 vs 2.38%; P < 0.05). A decrease in progesterone levels in the mated recipients before the due date induced natural delivery. However, cloned pups in non-mated recipients were delivered by Caesarean section because the fall in progesterone concentration in these females did not occur until the due date. The present study demonstrated for the first time that mated female dogs can be used as recipients for cloned embryos.
Additional keywords: embryo transfer, mated recipient, nuclear transfer.
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