Oocyte glutathione and fertilisation outcome of Macaca nemestrina and Macaca fascicularis in in vivo- and in vitro-matured oocytes
E. C. Curnow A B C D , J. P. Ryan B C , D. M. Saunders B C and E. S. Hayes A
+ Author Affiliations
- Author Affiliations
A Washington National Primate Research Center, University of Washington, Seattle, WA 98121, USA.
B Sydney Medical School, Edward Ford Building, A27, University of Sydney, NSW 2006, Australia.
C IVF Australia, 176 Pacific Highway, Greenwich, NSW 2065, Australia.
D Corresponding author. Email: ecurnow@wanprc.org
Reproduction, Fertility and Development 22(6) 1032-1040 https://doi.org/10.1071/RD09308
Submitted: 18 December 2009 Accepted: 1 February 2010 Published: 1 July 2010
Abstract
Fertilisation and development of IVM non-human primate oocytes is limited compared with that of in vivo-matured (IVO) oocytes. The present study describes the IVM of macaque oocytes with reference to oocyte glutathione (GSH). Timing of maturation, comparison of IVM media and cysteamine (CYS) supplementation as a modulator of GSH were investigated. A significantly greater proportion of oocytes reached MII after 30 h compared with 24 h of IVM. Following insemination, IVM oocytes had a significantly lower incidence of normal fertilisation (i.e. 2PN = two pronuclei and at least one polar body) and a higher rate of abnormal fertilisation (1PN = one pronucleus and at least one polar body) compared with IVO oocytes. Immunofluorescence of 1PN zygotes identified incomplete sperm head decondensation and failure of male pronucleus formation as the principal cause of abnormal fertilisation in IVM oocytes. The IVO oocytes had significantly higher GSH content than IVM oocytes. Cumulus-denuded oocytes had significantly lower GSH following IVM compared with immature oocytes at collection. Cysteamine supplementation of the IVM medium significantly increased the GSH level of cumulus-intact oocytes and reduced the incidence of 1PN formation, but did not improve GSH levels of the denuded oocyte. Suboptimal GSH levels in macaque IVM oocytes may be related to reduced fertilisation outcomes.
Additional keywords: cumulus-denuded oocyte, cysteamine, male pronucleus formation.
Acknowledgements
This work was supported by the National Center for Research Resources (P51 grant RR00166). The authors thank the Washington National Primate Research Center Tissue Distribution Program for their assistance with the collection of reproductive tissues, C. Astley and J. Aherns for surgery support and C. Ferrier for animal care.
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