Embryo biotechnology in the dog: a review
Sylvie Chastant-Maillard A B C E , Martine Chebrout B C , Sandra Thoumire B C , Marie Saint-Dizier B C D , Marc Chodkiewicz A B and Karine Reynaud B C
+ Author Affiliations
- Author Affiliations
A Department of Reproduction, Ecole Nationale Vétérinaire d’Alfort, 7 Avenue du Général De Gaulle, 94700 Maisons-Alfort, France.
B ENVA, UMR 1198 Biologie du Développement et Reproduction, 7 Avenue du Général De Gaulle, 94700 Maisons-Alfort, France.
C INRA, UMR 1198 Biologie du Développement et Reproduction, F-78350 Jouy en Josas, France.
D Genetic Breeding and Reproduction, AgroParisTech, 16 Rue Claude Bernard, 75005 Paris, France.
E Corresponding author. Email: schastant@vet-alfort.fr
Reproduction, Fertility and Development 22(7) 1049-1056 https://doi.org/10.1071/RD09270
Submitted: 4 November 2009 Accepted: 3 March 2010 Published: 20 August 2010
Abstract
Canine embryos are a scarce biological material because of difficulties in collecting in vivo-produced embryos and the inability, to date, to produce canine embryos in vitro. The procedure for the transfer of in vivo-produced embryos has not been developed adequately, with only six attempts reported in the literature that have resulted in the birth of 45 puppies. In vitro, the fertilisation rate is particularly low (∼10%) and the incidence of polyspermy particularly high. So far, no puppy has been obtained from an in vitro-produced embryo. In contrast, cloning of somatic cells has been used successfully over the past 4 years, with the birth of 41 puppies reported in the literature, a yield that is comparable to that for other mammalian species. Over the same period, canine embryonic stem sells and transgenic cloned dogs have been obtained. Thus, the latest reproductive technologies are further advanced than in vitro embryo production. The lack of fundamental studies on the specific features of reproductive physiology and developmental biology in the canine is regrettable in view of the increasing role of dogs in our society and of the current demand for new biological models in biomedical technology.
Additional keywords: biology, development, fertilisation, in vitro, in vivo, techniques.
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