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RESEARCH ARTICLE
Contents Vol 22(6)

Endocrine milieu of perioestrus in the giant panda (Ailuropoda melanoleuca), as determined by non-invasive hormone measures

David C. Kersey A D E , David E. Wildt A , Janine L. Brown A , Rebecca J. Snyder B , Yan Huang C and Steven L. Monfort A
+ Author Affiliations
- Author Affiliations

A Center for Species Survival, Smithsonian Conservation Biology Institute, Front Royal, VA 22630, USA.

B Zoo Atlanta, Atlanta, GA 30315, USA.

C China Conservation and Research Center for the Giant Panda, Wolong Nature Reserve, Wenchuan, Sichuan 623006, China.

D Present address: Western University of Health Sciences, College of Veterinary Medicine, Pomona, CA 91766, USA.

E Corresponding author. Email: dkersey@westernu.edu

Reproduction, Fertility and Development 22(6) 901-912 https://doi.org/10.1071/RD09178
Submitted: 29 July 2009  Accepted: 15 January 2010   Published: 25 May 2010

Abstract

The aim of the present study was to determine the efficacy of faecal hormonal measures for evaluating ovarian activity in a significant sized cohort of giant pandas during the perioestrual period. Faecal excretion of oestrogen and progestagen metabolites corresponded with urinary patterns and receptive behaviours. Longitudinal assessment of 10 females revealed that, on average, faecal oestrogen concentrations started to rise (P < 0.05) above baseline (baseline mean ± s.e.m.; 64.7 ± 6.6 ng g−1) 5 days before the preovulatory oestrogen peak (484.6 ± 126.8 ng g−1), which was followed by a gradual descent over 4 days to nadir. Mean faecal progestagen metabolite concentrations increased approximately twofold above baseline (from 186.2 ± 37.7 to 347.2 ± 75.7 ng g−1; P < 0.05) during the 20-day interval after the preovulatory oestrogen surge. Variability within and among females precluded the use of a threshold of oestrogen or progestagen metabolites to predict reproductive status, yet faeces collected 2–3 days per week provided sufficient data to recognise that an individual was in the perioestrual period. Finally, in females that were examined for at least 3 consecutive years, there was an 18–53 day variation in the onset and an 8–13 day variation in the duration of perioestrual behaviour from year to year. In summary, these findings indicate that gonadal hormone profiles associated with the period immediately before, during and after oestrus are accurately revealed by analysis of the fibrous faeces of the giant panda. This approach has potential value for providing point-in-time information on the reproductive status of free-living individuals.

Additional keywords: bear reproduction, faecal hormones.


Acknowledgements

The authors thank Susan Walker, Adrienne Crosier, Nicole Abbondanza, Karen Steinman, Copper Aitken-Palmer and Nicole Presley for logistical support. Special thanks to Nicole Savageau, Bridgette von Holdt, Jessica Beckman, Valerie Parkman, Serena Enloe and Corinna Bazlett for laboratory assistance. The authors also acknowledge the enormous contributions made by the keepers and curatorial staff at the Smithsonian National Zoological Park, Zoo Atlanta and the China Conservation and Research Center for the Giant Panda in terms of the frequent and accurate sample collection. This study was supported by The Friends of the National Zoo and the Smithsonian National Zoological Park.


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