Expression of follicle-stimulating hormone and luteinising hormone binding sites in the bitch ovary during the follicular phase
Marie Saint-Dizier A B E F , Nina Jaffré A E , Karine Reynaud A , Benoît Remy D , Sandra Thoumire A C and Sylvie Chastant-Maillard A CA UMR 1198 INRA/ENVA/CNRS Biologie du Développement et Reproduction, 7 Avenue du Général de Gaulle, 94 700 Maisons-Alfort, France.
B AgroParisTech (Paris Institute of Technology for Life, Food and Environmental Sciences), 16 rue Claude Bernard, 75 231 Paris Cedex 05, France.
C Department of Reproduction, Ecole Nationale Vétérinaire d’Alfort, 7 Avenue du Général de Gaulle, 94 700 Maisons-Alfort, France.
D Physiologie de la reproduction animale, Faculté de Médecine Vétérinaire, Université de Liège, Boulevard de Colonster 20, B-4000 Liège (Sart Tilman), Belgique.
E These authors contributed equally to this work.
F Corresponding author. Email: stdizier@agroparistech.fr
Reproduction, Fertility and Development 20(8) 925-934 https://doi.org/10.1071/RD08119
Submitted: 26 May 2008 Accepted: 21 July 2008 Published: 10 October 2008
Abstract
In the female dog, in contrast with most mammals, the growing follicle starts to luteinise several days before ovulation. Little is known about the physiological control of the final follicular growth in this species. In order to better understand the pituitary regulation of follicular growth, specific binding sites for FSH and LH were localised and quantified by autoradiography using [125I]-porcine (p) gonadotrophins on ovarian sections (7 μm) from adult Beagle bitches during the follicular phase. Follicles were analysed either before the LH surge (n = 4 bitches; n = 117 follicles) or after the LH surge and before ovulation (n = 5 bitches; n = 110 follicles). FSH binding sites were specifically and homogeneously expressed at high levels on granulosa cells of all healthy follicles from the preantral stage onwards. In contrast, LH binding sites were detected homogeneously and at high levels only on granulosa cells of follicles larger than 1 mm in diameter, including luteinised follicles. Theca binding of LH (but not FSH) was also observed, but only when using high concentrations of [125I]-pLH. The overall incidence of atresia was 45.8% and was dependent upon follicular diameter. Quantitative analysis of labelling showed that atretic follicles had reduced levels of both FSH and LH binding sites compared with healthy follicles. In healthy follicles, levels of both FSH and LH binding sites changed with follicle diameter. Compared with other mammals, the acquisition of LH binding on canine granulosa cells occurs in smaller sized follicles relative to the size of ovulation.
Acknowledgements
The authors thank Marc Chodkiewicz for critical reading of the manuscript. This work was funded by the National Institute for Agronomic Research (INRA) and the French Department of Agriculture and Fishing (Ministère de l’Agriculture et de la Pêche). The authors declare that there is no conflict of interest that would prejudice the impartiality of this study.
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