In vivo and in vitro effects of interleukin-1β on equine oocyte maturation and on steroidogenesis and prostaglandin synthesis in granulosa and cumulus cells
Maud Caillaud A B C D E and Nadine Gérard A B C D E F
+ Author Affiliations
- Author Affiliations
A INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France.
B CNRS, UMR 6175, F-37380 Nouzilly, France.
C Université François Rabelais, F-37041 Tours, France.
D Haras Nationaux, F-37380 Nouzilly, France.
E IFR 135, Imagerie fonctionnelle, F-37041 Tours, France.
F Corresponding author. Email: gerard@tours.inra.fr
Reproduction, Fertility and Development 21(2) 265-273 https://doi.org/10.1071/RD08046
Submitted: 13 March 2008 Accepted: 8 September 2008 Published: 27 January 2009
Abstract
We analysed the effect of interleukin-1 on oocyte maturation and on steroid and prostaglandin production by equine granulosa and cumulus cells. In Experiment 1, interleukin-1β (IL-1β) was injected into the growing dominant follicle, which was punctured 38 h later. Follicular fluid was assayed for steroids and prostaglandin-F2α (PGF2α). Granulosa cells were analysed for 3β-hydroxysteroid dehydrogenase (3β-HSD), progesterone receptor (PR), cyclooxygenase 1 and 2 (Cox 1 and Cox 2) and steroidogenic acute regulatory protein (StAR) mRNAs. In Experiment 2, cumulus–oocyte complexes (COCs) were collected from slaughterhouse ovaries and cultured in different media: control group (TCM199 + BSA); Group 2 (+ IL-1β); Group 3 (+ EGF); Group 4 (+ EGF + IL-1β); and Group 5 (+ EGF + IL-1β + IL-1RA). Cumulus cells were analysed for 3β-HSD, PR, Cox 1, Cox 2 and StAR mRNAs. After injections of crude equine gonadotropin (CEG; LH effect) or IL-1β, progesterone and PGF2α levels increased, whereas 17β-oestradiol decreased. EGF induced an increase in the rate of in vitro maturation (P < 0.05), whereas IL-1β had a limited effect. IL-1β significantly decreased the rate of EGF-induced oocyte maturation (P < 0.05). Cox 2 mRNA level increases in granulosa cells after CEG injection (P = 0.07). In cumulus cells, StAR and PR mRNAs were lower in Group 2 and 3β-HSD mRNA was higher in Groups 4 and 5. These data confirm that IL-1 is involved in equine oocyte in vitro maturation. We demonstrated in vivo that IL-1β has an effect on steroids and PGF2α secretion in the preovulatory follicle.
Additional keyword: mare.
Acknowledgements
The authors wish to thank Guy Duchamp for technical assistance and for his help during statistical analysis, and Cécile Lahuec, Vincent Jonchère, Bernard Bruneau and the staff of the experimental stud for technical assistance. We are grateful to Gaël Ramé for the collection of slaughterhouse ovaries. Liliane Martin is acknowledged for proof-reading and correction of English. This work was supported by grants from Institut National de la Recherche Agronomique, France, and the Haras Nationaux, France. Maud Caillaud was supported by a fellowship from the Haras Nationaux, France and the Région Centre, France.
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