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RESEARCH ARTICLE
Contents Vol 20(4)

Analysis of selected transcript levels in porcine spermatozoa, oocytes, zygotes and two-cell stage embryos

Bartosz Kempisty A , Paweł Antosik B , Dorota Bukowska B , Marta Jackowska A , Margarita Lianeri A , Jędrzej M. Jaśkowski B and Paweł P. Jagodziński A C
+ Author Affiliations
- Author Affiliations

A Department of Biochemistry and Molecular Biology, University of Medical Sciences, Święcickiego 6 St. 60-781, Poznań, Poland.

B Department of Agricultural Veterinary, University of Agriculture, Wojska Polskiego 52 St. 60-628, Poznań, Poland.

C Corresponding author. Email: pjagodzi@am.poznan.pl

Reproduction, Fertility and Development 20(4) 513-518 https://doi.org/10.1071/RD07211
Submitted: 20 November 2007  Accepted: 21 February 2008   Published: 11 April 2008

Abstract

It has been suggested that spermatozoa can deliver mRNAs to the oocyte during fertilisation. Using reverse transcription and real-time quantitative polymerase chain reaction analysis (RQ-PCR), we evaluated the presence of clusterin (CLU), protamine 2 (PRM2), calmegin (CLGN), cAMP-response element modulator protein (CREM), methyltransferase 1 (DNMT1), linker histone 1 (H1), protamine 1 (PRM1), TATA box-binding protein associated factor 1 (TAF1) and TATA box-binding protein (TBP) in porcine mature oocytes, zygotes and two-cell stage embryos. Spermatozoa isolated from semen samples of boars contained all transcripts investigated, whereas oocytes contained only CREM, H1, TAF1, and TBP mRNAs. The zygote and two-cell stage embryos contained CLU, CREM, H1, PRM1, PRM2, TAF1 and TBP transcripts. Our observations suggest that porcine spermatozoa may delivery CLU, PRM1 and PRM2 mRNAs to the oocyte, which may contribute to zygotic and early embryonic development.

Additional keywords: mRNA, pig.


Acknowledgements

This study was supported by grant no. 1682/B/PO1/2007/33 from the Polish Ministry of Scientific Research and Information Technology.


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