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Vertebrate reproductive science and technology
RESEARCH ARTICLE (Open Access)

Changes in cell cycle and extracellular matrix gene expression during placental development in deer mouse (Peromyscus) hybrids

Amanda R. Duselis A , Craig Obergfell B , Jennifer A. Mack B , Michael J. O’Neill B , Quang K. Nguyen A , Rachel J. O’Neill B and Paul B. Vrana A C
+ Author Affiliations
- Author Affiliations

A Department of Biological Chemistry, Sprague Hall 350, School of Medicine, University of California Irvine, Irvine, CA 92799-1700, USA.

B Department of Molecular & Cell Biology, University of Connecticut, BH 309A, 345 Mansfield Road, Unit 2131, Storrs, CT 06269-2131, USA.

C Corresponding author. Email: pvrana@uci.edu

Reproduction, Fertility and Development 19(5) 695-708 https://doi.org/10.1071/RD07015
Submitted: 21 January 2007  Accepted: 6 May 2007   Published: 4 July 2007

Abstract

Crosses between two species of the rodent genus Peromyscus produce defects in both growth and development. The defects are pronounced in the hybrid placentas. Peromyscuys maniculatus (strain BW) females mated to P. polionotus (strain PO) males produce placentas half the size of the parental species, as well as growth-retarded embryos. In contrast, PO females mated to BW males result in defective conceptuses that display embryonic and placental overgrowth. These ‘parent-of-origin’-dependent phenotypes are consistent with previous studies that demonstrated altered expression of imprinted genes and genetic linkage of the overgrowth phenotypes to imprinted domains. In the present study, we take a broader approach in assessing perturbations in hybrid placental gene expression through the use of Mus musculus cDNA microarrays. In verifying classes of genes identified in microarray screens differentially regulated during hybrid placental development, we focused on those influencing the cell cycle and extracellular matrix (ECM). Our work suggests that cell cycle regulators at the G1/S phase check-point are downregulated in the large hybrid placenta, whereas the small hybrid placenta is more variable. The ECM genes are typically downstream targets of cell cycle regulation and their misregulation is consistent with many of the dysmorphic phenotypes. Thus, these data suggest imbalances in proliferation and differentiation in hybrid placentation.


Acknowledgements

This research was supported by grants from the American Cancer Society (RSG-03–070–01-MGO) and March of Dimes (#5-FY03–17) to PBV, the National Science Foundation (MCB-0093250) to RJO and the University of Connecticut Research Foundation to RJO and MJO. The authors thank the Center for Applied Genetics and Technology for Affymetrix support and the Center for Molecular and Mitochondrial Medicine and Genetics for assistance with microscopy and imaging.


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