Effects of repetition, interval between treatments and season on the results from laparoscopic ovum pick-up in goats
J. Pierson A , B. Wang A , N. Neveu A , L. Sneek A , F. Côté A , C. N. Karatzas A and H. Baldassarre A BA Nexia Biotechnologies Inc., 320 Chemin St Georges, St Telesphore, Quebec, J0P 1G0, Canada.
B Corresponding author. Email: hbaldassarre@nexiabiotech.com
Reproduction, Fertility and Development 16(8) 795-799 https://doi.org/10.1071/RD04066
Submitted: 29 June 2004 Accepted: 24 October 2004 Published: 13 January 2005
Abstract
The present study was conducted to evaluate the follicular response and oocyte yield following repeated gonadotrophin stimulation and laparoscopic aspiration in goats and to assess the effects of the time interval between procedures and season. A total of 98 adult goats were subjected to laparoscopic ovum pick-up (LOPU) five consecutive times in a transgenic production programme. Oestrus was synchronised by means of intravaginal sponges inserted for 10 days coupled with 125 μg cloprostenol 36 h before sponge removal and LOPU, and follicular development was stimulated with 80 mg follicle stimulating hormone and 300 IU equine chorionic gonadotrophin administered 36 h before LOPU. No difference was detected in the response for LOPUs 1, 2, 3 and 4. Although a small decrease in response was detected at LOPU 5 (P < 0.05), the numbers of follicles aspirated and oocytes recovered were not different from those at LOPU 1 and LOPUs 1 and 4, respectively. With respect to time interval between LOPU and season, all intervals and seasons produced acceptable responses, with no difference in follicles aspirated and oocytes recovered between intervals and seasons. These results indicate that LOPU may be repeated up to five times in goats at different intervals and in different seasons with little or no important change in overall response.
Extra keywords: folliculocentesis, gonadotrophin treatment, oocyte aspiration.
Baldassarre, H. , and Karatzas, C. N. (2004). Advanced assisted reproduction technologies (ART) in goats. Anim. Reprod. Sci. 82–83, 255–266.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Baldassarre, H. , de Matos, D. G. , Furnus, C. C. , Castro, T. E. , and Cabrera Fischer, E. I. (1994). Technique for efficient recovery of sheep oocytes by laparoscopic folliculocentesis. Anim. Reprod. Sci. 35, 145–150.
| Crossref | GoogleScholarGoogle Scholar |
Baldassarre, H. , Furnus, C. C. , de Matos, D. G. , and Pessi, H. (1996). In vitro production of sheep embryos using laparoscopic folliculocentesis: alternative gonadotrophin treatments for stimulation of oocyte donors. Theriogenology 45, 707–717.
| Crossref | GoogleScholarGoogle Scholar |
Baldassarre, H. , Keefer, C. L. , Gauthier, M. , Bhatia, B. , Begin, I. , Pierson, J. , Laurin, D. , Trigg, T. , Downey, B. R. , and Karatzas, C. N. (2001). Laparoscopic ovum pick-up and zygote recovery in goats treated with deslorelin implants before superovulation. Theriogenology 55, 510.[Abstract]
Baldassarre, H. , Wang, B. , Kafidi, N. , Keefer, C. L. , Lazaris, A. , and Karatzas, C. N. (2002). Advances in the production and propagation of transgenic goats using laparoscopic ovum pick-up and in vitro embryo production technologies. Theriogenology 57, 275–284.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Baldassarre, H. , Wang, B. , Kafidi, N. , Gauthier, M. , and Neveu, N. , et al. (2003a). Production of transgenic goats by pronuclear microinjection of in vitro produced zygotes from oocytes recovered by laparoscopy. Theriogenology 59, 831–839.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Baldassarre, H. , Keefer, C. , Wang, B. , Lazaris, A. , and Karatzas, C. N. (2003b). Nuclear transfer in goats using in vitro matured oocytes recovered by laparoscopic ovum pick-up. Cloning Stem Cells 5, 279–285.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Cognié, Y. (1999). State of the art in sheep–goat embryo transfer. Theriogenology 51, 105–116.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Cognié, Y. , Baril, G. , Poulin, N. , and Mermillod, P. (2003). Current status of embryo technologies in sheep and goat. Theriogenology 59, 171–188.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Drion, P. V. , Furtoss, V. , Baril, G. , Manfredi, E. , and Bouvier, F. , et al. (2001). Four years of induction/synchronization of estrus in dairy goats: effect on the evolution of eCG binding in relation with the parameters of reproduction. Reprod. Nutr. Dev. 41, 401–412.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Gonzalez-Bulnes, A. , Garcia-Garcia, R. M. , Santiago-Moreno, J. , Dominguez, V. , Lopez-Sebastian, A. , and Cocero, M. J. (2003). Reproductive season affects inhibitory effects from large follicles on the response to superovulatory FSH treatments in ewes. Theriogenology 60, 281–288.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Graff, K. J. , Meintjes, M. , Paul, J. B. , Dyer, V. W. , Denniston, R. S. , Ziomek, C. A. , and Godke, R. A. (1995). Ultrasound-guided transvaginal oocyte recovery from FSH-treated goats for IVF. Theriogenology 43, 223.[Abstract]
| Crossref | GoogleScholarGoogle Scholar |
Graff, K. J. , Meintjes, M. , Dyer, V. W. , Paul, J. B. , Denniston, R. S. , Ziomek, C. A. , and Godke, R. A. (1999). Transvaginal ultrasound-guided oocyte retrieval following FSH stimulation of domestic goats. Theriogenology 51, 1099–1119.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Koeman, J. , Keefer, C. L. , Baldassarre, H. , and Downey, B. R. (2003). Developmental competence of prepubertal and adult goat oocytes cultured in semi-defined media following laparoscopic recovery. Theriogenology 60, 879–889.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Pintado, B. , Gutierrez-Adan, A. , and Perez Llano, B. (1998). Superovulatory response of Murciana goats to treatments based on PMSG/Anti-PMSG or combined FSH/PMSG administration. Theriogenology 50, 357–364.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Remy, B. , Baril, G. , Vallet, J. C. , Dufour, R. , Chouvet, C. , Saumande, J. , Chupin, D. , and Beckers, J. F. (1991). Are antibodies responsible for a decreased superovulatory response in goats which have been treated repeatedly with pFSH? Theriogenology 36, 389–399.
| Crossref | GoogleScholarGoogle Scholar |
Roy, F. , Maurel, M. C. , Combes, B. , Vaiman, D. , Cribiu, E. P. , Lantier, I. , Pobel, T. , Delétang, F. , Combarnous, Y. , and Guillou, F. (1999). The negative effect of repeated equine chorionic gonadotrophin treatment on subsequent fertility in Alpine goats is due to a humoral immune response involving the major histocompatibility complex. Biol. Reprod. 60, 805–813.
| PubMed |
Snyder, D. A. , and Dukelow, R. (1974). Laparoscopic studies of ovulation, pregnancy diagnoses, and follicle aspiration in sheep. Theriogenology 2, 143–148.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Stangl, M. , Kühholzer, B. , Besenfelder, U. , and Brem, G. (1999). Repeated endoscopic ovum pick-up in sheep. Theriogenology 52, 709–716.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Tervit, H. R. (1996). Laparoscopy/laparotomy oocyte recovery and juvenile breeding. Anim. Reprod. Sci. 42, 227–238.
| Crossref | GoogleScholarGoogle Scholar |
