Major plasma proteins in pig serum during postnatal development
M. Martin A , M. A. Tesouro B , N. González-Ramón C , A. Piñeiro C and F. Lampreave C DA Junta de Castilla y León, Centro de Salud, Lerma, Burgos, Spain.
B Departamento de Patología Animal (Medicina Veterinaria), Facultad de Veterinaria de León, Spain.
C Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Spain.
D Corresponding author. Email: lampreav@unizar.es
Reproduction, Fertility and Development 17(4) 439-445 https://doi.org/10.1071/RD04064
Submitted: 25 June 2004 Accepted: 6 February 2005 Published: 23 March 2005
Abstract
The time-course of changes in the levels of albumin, α-fetoprotein (AFP), α1-protease inhibitor (α1-antitrypsin), α1-acid glycoprotein, fetuin, haptoglobin, transferrin, IgG and the major acute-phase protein (Pig-MAP) in the blood sera of pigs during the first days and weeks of life was investigated by quantitative radial immunodiffusion. The serum of newborn pigs before suckling was characterised by a very low concentration of total proteins (approximately 25 mg mL–1), low levels of albumin and transferrin and the lack of immunoglobulins. In contrast, α1-acid glycoprotein and fetuin are present at high levels (approximately 12 and 5 mg mL–1 respectively). The results of the present study show that the piglets undergo a very rapid metabolic maturation with regard to serum proteins, evolving from a characteristic ‘fetal’ pattern to an ‘adult’ one. We have paid special attention to the evolution of haptoglobin and Pig-MAP, which are two important acute-phase proteins in pigs. The evolution of serum levels of these proteins suggests that piglets must overcome a moderate acute-phase situation during the first week of life.
Extra keywords: acute-phase proteins, haptoglobin, inter-α-trypsin inhibitor heavy chain 4, major acute-phase protein (Pig-MAP), neonatal pigs.
Cavanagh, M. E. , Cornelis, M. E. , Dziegielewska, K. M. , Luft, A. J. , Lai, P. C. W. , Lorscheider, F. L. , and Saunders, N. R. (1982). Proteins in cerebrospinal fluid and plasma of fetal pigs during development. Dev. Neurosci. 5, 492–502.
| PubMed |
Galembeck, F. , and Cann, J. R. (1974). Fetuin as a trypsin inhibitor. Arch. Biochem. Biophys. 164, 326–331.
| PubMed |
González-Ramón, N. , Alava, M. A. , Sarsa, J. A. , Piñeiro, M. , Escartin, A. , García-Gil, A. , Lampreave, F. , and Piñeiro, A. (1995). The major acute phase serum protein in pigs is homologous to human plasma kallikrein sensitive PK-120. FEBS Lett. 371, 227–230.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
![](../../media/share/OpenURL_Image.gif)
Hall, W. F. , Eurell, T. E. , Hansen, R. D. , and Herr, L. G. (1992). Serum haptoglobin concentration in swine naturally or experimentally infected with Actinobacillus pleuropneumoniae. J. Am. Vet. Med. Assoc. 201, 1730–1733.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Heegaard, P. M. H. , Klausen, J. , Nielsen, J. P. , González-Ramón, N. , Piñeiro, M. , Lampreave, F. , and Alava, M. A. (1998). The porcine acute phase response to infection with Actinobacillus pleuropneumoniae. Haptoglobin, C-reactive protein, major acute phase protein and serum amyloid A protein are sensitive indicators of infection. Comp. Biochem. Physiol. 119B, 365–373.
![](../../media/share/OpenURL_Image.gif)
Huang, L. , Yoneda, M. , and Kimata, K. (1993). A serum-derived hyaluronan-associated protein (SHAP) is the heavy chain of the inter-alpha-trypsin inhibitor. J. Biol. Chem. 268, 26 725–26 730.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Ingvarsson, B. I. , Carlsson, R. N. K. , and Karlsson, B. W. (1978). Synthesis of α-fetoprotein, albumin and total serum protein in neonatal pigs. Biol. Neonate 34, 259–268.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Itoh, H. , Tamura, K. , Izumi, M. , Motoi, Y. , and Funayama, Y. (1993a). Characterization of serum α1-acid glycoprotein in fetal and newborn calves during development. Am. J. Vet. Res. 54, 591–595.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Itoh, H. , Tamura, K. , Izumi, M. , Motoi, Y. , Kidoguchi, K. , and Funayama, Y. (1993b). The influence of age and health status on the serum α1-acid glycoprotein level of conventional and specific pathogen-free pigs. Can. J. Vet. Res. 57, 74–78.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Karlsson, B. W. (1970). Fetoprotein and albumin levels in the blood serum of developing neonatal pigs. Comp. Biochem. Physiol. 34, 823–827.
![](../../media/share/OpenURL_Image.gif)
Klobasa, F. , Werhahn, E. , and Butler, J. E. (1981). Regulation of humoral immunity in the piglet by immunoglobulins of maternal origin. Res. Vet. Sci. 31, 195–206.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Kushner, I. (1982). The phenomenon of the acute phase response. Ann. N.Y. Acad. Sci. 389, 39–48.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Lampreave, F. , and Piñeiro, A. (1982). Characterisation of a new alpha-glycoprotein as the major serum component in later fetal and newborn pigs. Comp. Biochem. Physiol. 72B, 215–219.
![](../../media/share/OpenURL_Image.gif)
Lampreave, F. , and Piñeiro, A. (1984). The major serum protein of fetal and newborn pigs: biochemical properties and identification as a fetal form of α1-acid glycoprotein. Int. J. Biochem. 16, 47–53.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
![](../../media/share/OpenURL_Image.gif)
Lampreave, F. , and Piñeiro, A. (1992). Concentrations of major plasma proteins in serum and whole-tissue extracts of porcine fetuses during development. J. Reprod. Fertil. 95, 441–449.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Lampreave, F. , González-Ramón, N. , Martínez-Ayensa, S. , Hernández, M. A. , Lorenzo, H. K. , García-Gil, A. , and Piñeiro, A. (1994). Characterisation of the acute phase serum protein response in pigs. Electrophoresis 15, 672–676.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Lowry, O. H. , Rosebrough, N. J. , Farr, A. L. , and Randall, R. J. (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Mancini, G. , Carbonara, A. O. , and Heremans, J. F. (1965). Immunochemical quantification of antigens by single radial immunodiffusion. Immunochemistry 2, 235–254.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
![](../../media/share/OpenURL_Image.gif)
Nishimura, H. , Kakizaki, I. , Muta, T. , Sasaki, N. , Pu, X. P. , Yamashita, T. , and Nagasawa, S. (1995). cDNA and deduced amino acid sequence of human PK-120, a plasma kallikrein-sensitive glycoprotein. FEBS Lett. 357, 207–211.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
![](../../media/share/OpenURL_Image.gif)
Saguchi, K. , Tobe, T. , Hashimoto, K. , Sano, Y. , Nakano, Y. , Miura, N.-H. , and Tomita, M. (1995). Cloning and characterisation of cDNA for inter-α-trypsin inhibitor family heavy chain-related protein (IHRP), a novel human plasma glycoprotein. J. Biochem. 117, 14–18.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Salier, J. P. , Rouet, P. , Raguenez, G. , and Daveau, M. (1996). The inter-α-inhibitor family: from structure to regulation. Biochem. J. 315, 1–9.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Stone, R. T. (1981). In vitro liver synthesis and serum levels of alpha-fetoprotein and albumin in the fetal pig. Biol. Reprod. 20, 573–580.
![](../../media/share/OpenURL_Image.gif)
Stone, R. T. (1989). Isolation and characterisation of recombinant cDNA clones corresponding to developmentally regulated genes in pig liver. J. Anim. Sci. 67, 1082–1089.
| PubMed |
![](../../media/share/OpenURL_Image.gif)
Thoren-Tolling, V. , and Martinsson, K. (1974). On the transferrin concentration in serum of sows and growing pigs and in sow colostrum. Acta Vet. Scand. 15, 1120–1134.
![](../../media/share/OpenURL_Image.gif)
Travis, J. , and Salveson, G. S. (1983). Human plasma proteinase inhibitors. Annu. Rev. Biochem. 52, 655–709.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
![](../../media/share/OpenURL_Image.gif)
Weström, B. R. , Karlsson, B. W. , and Svendsen, J. (1982). Levels of serum protease inhibitors during fetal and postnatal development of the pig. Biol. Neonate 41, 22–31.
| PubMed |
![](../../media/share/OpenURL_Image.gif)