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RESEARCH ARTICLE
Contents Vol 15(4)

Effect of ovarian steroids on basal and oxytocin-induced prostaglandin F2α secretion from pig endometrial cells

Jianbo Hu A B E , Gheorghe T. Braileanu A C and Mark A. Mirando A D
+ Author Affiliations
- Author Affiliations

A Department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164-6353, USA.

B Present address: Department of Animal Science, Texas A&M University, College Station, TX 77843-2471, USA.

C Present address: Department of Pediatrics, School of Medicine, University of Maryland, Baltimore, MD 21201, USA.

D Present address: National Research Initiative Grants Program, 1400 Independence Avenue, S. W. Stop 2241, US Department of Agriculture, Washington, DC 20250-2241, USA.

E To whom correspondence should be addressed. email: jianbo@tamu.edu

Reproduction, Fertility and Development 15(4) 197-205 https://doi.org/10.1071/RD03024
Submitted: 4 March 2003  Accepted: 9 May 2003   Published: 13 August 2003

Abstract

These studies were undertaken to determine how treatment with 100 nM progesterone and/or 10 nM oestradiol-17β acutely (3 h; Experiment 1) or chronically (72 h; Experiments 2–4) influenced basal and oxytocin (OT)-stimulated prostaglandin (PG) F2α secretion, in enriched cultures of pig endometrial luminal epithelial, glandular epithelial and stromal cells obtained on Day 16 (Experiments 1, 2 and 4) or Day 12 (Experiment 3) after oestrus. In Experiment 1, acute treatment with progesterone stimulated PGF2α secretion from each cell type on Day 16, whereas acute oestradiol treatment inhibited the stimulatory action of progesterone on PGF2α secretion only in glandular epithelial cells. In Experiment 2, OT stimulated phospholipase (PL) C activity in luminal epithelial cells on Day 16 only in the presence of chronic oestradiol treatment. For glandular epithelial cells on Day 16, OT stimulated PLC activity only in the presence of chronic treatment with steroid. In stromal cells on Day 16, OT stimulated PLC activity in the absence of steroids and the response to OT was further enhanced by oestradiol. In the absence of chronic treatment with steroid, OT did not stimulate PGF2α secretion from luminal epithelial cells, but oestradiol induced a response to OT. For glandular epithelial cells, OT-induced PGF2α secretion was not altered by steroids, whereas the stimulatory response to OT was inhibited by oestradiol or progesterone in stromal cells. For endometrial cells obtained on Day 12 after oestrus in Experiment 3, OT only stimulated PGF2α release from glandular epithelial and stromal cells. For luminal epithelial cells obtained on Day 16 after oestrus and cultured under polarizing conditions in Experiment 4, secretion of PGF2α occurred preferentially from the basolateral surface and was stimulated by OT more from the basolateral surface than from the apical surface. Oxytocin-induced PGF2α secretion from the apical surface was enhanced by chronic treatment with oestradiol, whereas that from the basolateral surface was enhanced by chronic treatment with progesterone. In summary, oestradiol enhanced OT-induced PGF2α secretion from the apical surface of luminal epithelial cells and reduced the response of stromal cells to OT, actions that may contribute to the reorientation of PGF2α from endocrine secretion (i.e. towards the uterine vasculature) to exocrine secretion (i.e. towards the uterine lumen) during pregnancy recognition in pigs.

Extra keywords: endometrium


Acknowledgments

The authors are indebted to Dr Fuller W. Bazer (Texas A&M University) for critical review of this manuscript and to Dr William W. Thatcher (University of Florida) for supplying the antiserum to PGF2α. The authors are also grateful to the members of M. A. Mirando’s laboratory for assistance with surgery and to the staff of the Washington State University Swine Center and the Experimental Animal Laboratory Building for assistance with the care and handling of animals. This work was supported by National Institutes of Health grant HD30268 to MAM.


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