
%0 Journal Article
%A Choi, S. H. 
%A Cho, S. R. 
%A Han, M. H. 
%A Kim, H. J. 
%A Choe, C. Y. 
%A Son, D. S. 
%A Woo, J. S. 
%A Sang, B. D. 
%A Chung, Y. G. 
%A Hoshi, H. 
%T 175 DETECTION OF LIPID GRANULES ON IVF BLASTOCYSTS MATURED IN APO-TRANSFERRIN IN KOREAN NATIVE COWS (HANWOO)
%J Reproduction, Fertility and Development
%D 2006/12/12
%V 19
%N 1
%P 204-204
%K 
%R https://doi.org/10.1071/RDV19N1AB175
%U https://www.publish.csiro.au/paper/RDV19N1AB175 
%X Apo-transferrin (apo-Tf) is found in mammalian sera and plays a role as an anti-oxidant in media. The objective was to investigate the effects of apo-Tf on in vitro maturation and development and to examine the characteristics of blastocysts derived from IVF production in the presence of apo-Tf in Korean native cows (Hanwoo). Ovaries were collected from a slaughterhouse and cumulus&#8211;oocyte complexes (COCs) were taken from 2&#8211;6-mm antral follicles. The collected COCs were washed 3 times with 0.1 M PVA-TCM-199 and matured in 10 µg mL-1 apo-Tf with TCM-199 at 39°C, 5% CO2, 95% air for 24 h. Matured COCs were fertilized with frozen&#8211;thawed Korean native cattle semen treated with BO medium containing 5 mM caffeine and 1 µg mL-1 heparin for 8 h, and developed to the blastocyst stage in 5% FBS, 0.3% BSA in TCM-199, and IVMD (IFP, Japan). To detect the lipid granules, blastocysts were fixed with 10% formalin-PBS for 2 h and then 50% ethanol for 2 min. Staining of blastocysts was performed with 1% Sudan Black B in 70% ethanol for 2 min. IVM and IVF were replicated 3 times. The results of blastocyst formation were analyzed by chi-squared test. The maturation rate was 92.6% at Met II in 10 µg mL-1 apo-Tf. The blastocyst formation was 22.5%, 38.4%, and 34.8% in 5% FBS (control), 0.3% BSA in TCM-199 (P < 0.05), and IVMD (P < 0.05), respectively. The lipid granules in blastocysts from the 5% FBS-TCM-199 group were larger than those in the 0.3% BSA-TCM-199 and IVMD groups. The results suggest that apo-Tf is an important factor for in vitro maturation and that FBS in culture medium affects lipid granule formation on bovine blastocysts derived from in vitro fertilization. 
