
TY  - JOUR
A1  - Marshall, JT 
A1  - Nancarrow, CD 
A1  - Brownlee, AG 
T1  - Cloning and sequencing of a cDNA encoding an ovine oestrus-associated oviducal protein
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1996
VL  - 8
IS  - 2
SP  - 305
EP  - 310
KW  - 
UR  - https://doi.org/10.1071/RD9960305 
N2  - Ovine oestrus-associated oviducal glycoprotein (oEGP) is synthesized and secreted specifically by the ampullary region of the ovine oviduct during the peri-ovulatory stages of the oestrous cycle. A cDNA that encodes oEGP was isolated and sequenced. Isolation of oEGP was achieved using the polymerase chain reaction (PCR) with primers based on a bovine oestrus-associated oviducal glycoprotein cDNA (bOGP) sequence. A 1599-bp cDNA encodes, in part, a deduced 519-amino acid sequence of mature protein which carries two potential N-linked glycosylation sites. The deduced amino acid sequence is more than 95% identical to that of bOGP and more than 74% identical to the first 491 amino acids of human oestrogen-dependent oviducal glycoprotein (hOGP). Northern blot hybridizations of RNA from several sheep tissues detected mRNA (2.4 kb) only in an ampulla oviduct sample. 
ER  -

