
%0 Journal Article
%A Marshall, JT 
%A Nancarrow, CD 
%A Brownlee, AG 
%T Cloning and sequencing of a cDNA encoding an ovine oestrus-associated oviducal protein
%J Reproduction, Fertility and Development
%D 1996/03/01
%V 8
%N 2
%P 305-310
%K 
%R https://doi.org/10.1071/RD9960305
%U https://www.publish.csiro.au/paper/RD9960305 
%X Ovine oestrus-associated oviducal glycoprotein (oEGP) is synthesized and secreted specifically by the ampullary region of the ovine oviduct during the peri-ovulatory stages of the oestrous cycle. A cDNA that encodes oEGP was isolated and sequenced. Isolation of oEGP was achieved using the polymerase chain reaction (PCR) with primers based on a bovine oestrus-associated oviducal glycoprotein cDNA (bOGP) sequence. A 1599-bp cDNA encodes, in part, a deduced 519-amino acid sequence of mature protein which carries two potential N-linked glycosylation sites. The deduced amino acid sequence is more than 95% identical to that of bOGP and more than 74% identical to the first 491 amino acids of human oestrogen-dependent oviducal glycoprotein (hOGP). Northern blot hybridizations of RNA from several sheep tissues detected mRNA (2.4 kb) only in an ampulla oviduct sample. 
