
TY  - JOUR
A1  - Carolina, Pustrovh 
A1  - Jawerbaum, Alicia 
A1  - Debora, Sinner 
A1  - Christian, Perotti 
A1  - Gimeno, Martha A. F. 
A1  - Gonzalez, Elida T. 
T1  - Diminished levels of prostaglandin E in type I diabetic oocyte&#8211;cumulus complexes. Influence of nitric oxide and superoxide dismutase
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1999
VL  - 11
IS  - 2
SP  - 105
EP  - 110
KW  - 
UR  - https://doi.org/10.1071/RD99033 
N2  - In the present work the prostaglandin E (PGE) production by ovulated, immature and in vitromatured oocyte&#8211;cumulus complexes (OCC) was evaluated in a rat model of type I diabetes induced by streptozotocin (60 mg kg&#8211;1). A diminished number of ovulated OCC were found in the type I diabetic rat. In contrast to the increment in PGE generation found previously in OCC and embryos from type II diabetic rats, it was found that PGE production by type I diabetic OCC was diminished in comparison with the controls. Nitric oxide synthase (NOS) activity is enhanced in proestrous ovaries from type I diabetic rats, but cGMP levels are diminished. SIN-1 (300 &#181;&#x041C;), a nitric oxide donor, significantly enhanced PGE generation by control OCC, but was unable to modify the PGE levels in type I diabetic OCC. L-NMMA, a nitric oxide inhibitor that diminished PGE values in type II diabetic OCC, did not modify PGE generation in either control and type I diabetic OCC. Superoxide dismutase (SOD, 1000 U mL&#8211;1), and SOD (1000 U mL&#8211;1) plus SIN-1 (300 &#181;&#x041C;), enhanced PGE generation by both control and diabetic OCC. The present results suggest that even when nitric oxide (NO) is overproduced in diabetic ovaries, the NO&#8211;PGE pathway is impaired in type I diabetic OCC. As SOD additions are able to increase PGE generation by diabetic OCC, high concentrations of free oxygen radicals might be quenching the NO, impairing its physiological functions. 
ER  -

