
%0 Journal Article
%A Beebe, LF 
%A Kaye, PL 
%T Preimplantation development in the streptozotocin-induced diabetic mouse
%J Reproduction, Fertility and Development
%D 1990/08/01
%V 2
%N 4
%P 407-412
%K 
%R https://doi.org/10.1071/RD9900407
%U https://www.publish.csiro.au/paper/RD9900407 
%X Streptozotocin (STZ) was used to develop a diabetic mouse model in which to study the development of the preimplantation embryo. STZ doses of 0, 160, 190, 210 and 240 mg kg-1 were given; 190 mg kg-1 was found to be the most suitable as the standard diabetogenic dose, providing about 60% mice with plasma glucose greater than 20 mM. The STZ-diabetic mice responded to superovulation with 10 i.u. of gonadotrophin in the same manner as control mice, producing similar embryo numbers at 48 h, 72 h and 96 h post-hCG. Furthermore, the proportion of 2-cell embryos collected from STZ-diabetic mice which developed to blastocysts in vitro was similar to that of 2-cell embryos from control mice. The STZ-diabetic mouse model after superovulation thus produced normal early preimplantation embryos whose development can be examined in detail in a diabetic environment. 
