
TY  - JOUR
A1  - Garnier, J. N. 
A1  - Green, D. I. 
A1  - Pickard, A. R. 
A1  - Shaw, H. J. 
A1  - Holt, W. V. 
T1  - Non-invasive diagnosis of pregnancy in wild black rhinoceros (<emph type="2">Diceros bicornis minor</emph>) by faecal steroid analysis
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1999
VL  - 10
IS  - 6
SP  - 451
EP  - 458
KW  - 20&#945;-dihydroprogesterone enzyme immunoassay, reproductive success.
UR  - https://doi.org/10.1071/RD98118 
N2  - The objective of this study was to determine whether faecal progestagen measurement could be used to diagnose pregnancy in wild black rhinoceros cows. Immunoreactive 20&#945;-progestagens were measured in faecal samples collected regularly (one or two times times per week) from pregnant and non-pregnant wild black rhinoceros females (n =6) in Zimbabwe. Fresh dung piles deposited by the study animals were serially sampled during prolonged periods of tracking with local game scouts. Samples were stored frozen, and dried prior to methanol extraction. Immunoreactivity in faecal extracts was measured with a 20&#945;-dihydroprogesterone enzyme immunoassay and was shown to reflect circulating progesterone concentrations. Mean concentrations of faecal 20&#945;-progestagens during each month of gestation were significantly higher than faecal concentrations in non-pregnant animals (P&#60;0.05), except during the second month of gestation. Faecal 20&#945;-progestagens remained 5&#8211;10 times higher than concentrations in non-pregnant animals from the 4th to 15th month of gestation. It was concluded that regular non-invasive reproductive monitoring of black rhinoceros in the wild was possible and that pregnancy could be accurately diagnosed from the measurement of 20&#945;-progestagens in faecal samples. The use of this technique in wild black rhinoceros populations will offer new perspectives for in situ management of this endangered species. 
ER  -

