
TY  - JOUR
A1  - Campbell, Lachlan 
A1  - Clulow, John 
A1  - Howe, Belinda 
A1  - Upton, Rose 
A1  - Doody, Sean 
A1  - Clulow, Simon 
T1  - <i>Corrigendum to</i>: Efficacy of short-term cold storage prior to cryopreservation of spermatozoa in a threatened lizard
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 2021
VL  - 33
IS  - 9
SP  - 619
EP  - 619
KW  - 
UR  - https://doi.org/10.1071/RD20231_CO 
N2  - Assisted reproductive technologies (ARTs) have a significant role to play in reptile conservation, yet are severely lacking. Previous attempts to cryopreserve spermatozoa in the threatened lizard Varanus panoptes achieved approximately 48% motile sperm post-thaw for samples frozen immediately after collection. However, the feasibility of extended cold storage before cryopreservation has not been tested. We held V. panoptes spermatozoa at either 25&#xb0;C or 4&#xb0;C for 8 days, assessing sperm motility at days 1, 2, 4 and 8. Subsamples were cryopreserved on days 1 and 4 following the previously reported protocol for this species. Percentage motility decreased rapidly at 25&#xb0;C, but did not decrease significantly until 4 days after collection at 4&#xb0;C, with >30% motility maintained after 8 days. There was no significant difference in post-thaw motility or viability of samples cryopreserved after 1 or 4 days storage at 4&#xb0;C, yielding substantial results for both parameters (mean motility 23.8% and 28.1% and mean viability 50.1% and 57.5% after 1 and 4 days respectively). We demonstrate the capacity to extend sperm viability for up to 8 days in unfrozen samples and to produce acceptable post-thaw motility in samples frozen after 4 days of storage, contributing to the development of valuable ARTs for lizards and other reptiles. 
ER  -

