
%0 Journal Article
%A Campbell, Lachlan 
%A Clulow, John 
%A Howe, Belinda 
%A Upton, Rose 
%A Doody, Sean 
%A Clulow, Simon 
%T <i>Corrigendum to</i>: Efficacy of short-term cold storage prior to cryopreservation of spermatozoa in a threatened lizard
%J Reproduction, Fertility and Development
%D 2021/07/20
%V 33
%N 9
%P 619-619
%K 
%R https://doi.org/10.1071/RD20231_CO
%U https://www.publish.csiro.au/paper/RD20231_CO 
%X Assisted reproductive technologies (ARTs) have a significant role to play in reptile conservation, yet are severely lacking. Previous attempts to cryopreserve spermatozoa in the threatened lizard Varanus panoptes achieved approximately 48% motile sperm post-thaw for samples frozen immediately after collection. However, the feasibility of extended cold storage before cryopreservation has not been tested. We held V. panoptes spermatozoa at either 25&#xb0;C or 4&#xb0;C for 8 days, assessing sperm motility at days 1, 2, 4 and 8. Subsamples were cryopreserved on days 1 and 4 following the previously reported protocol for this species. Percentage motility decreased rapidly at 25&#xb0;C, but did not decrease significantly until 4 days after collection at 4&#xb0;C, with >30% motility maintained after 8 days. There was no significant difference in post-thaw motility or viability of samples cryopreserved after 1 or 4 days storage at 4&#xb0;C, yielding substantial results for both parameters (mean motility 23.8% and 28.1% and mean viability 50.1% and 57.5% after 1 and 4 days respectively). We demonstrate the capacity to extend sperm viability for up to 8 days in unfrozen samples and to produce acceptable post-thaw motility in samples frozen after 4 days of storage, contributing to the development of valuable ARTs for lizards and other reptiles. 
