
%0 Journal Article
%A Nikolova, Diana B. 
%A Martinova, Yordanka S. 
%A Seidensticker, Martin 
%A Bellvé, Anthony R. 
%T Leukaemia inhibitory factor stimulates proliferation of prospermatogonial stem cells
%J Reproduction, Fertility and Development
%D 1998/04/23
%V 9
%N 7
%P 717-722
%K seminiferous growth factor (SGF).
%R https://doi.org/10.1071/R96116
%U https://www.publish.csiro.au/paper/R96116 
%X The effect of leukaemia inhibitory factor (LIF) on the proliferation of prospermatogonial stem cells was testedin vitro. Pieces of 3-day-old rat testis were cultured in the presence of a range of doses of LIF alone or in combination with 1 ng mL -1 seminiferous growth factor (SGF). Stimulation of the proliferative activity of quiescent prospermatogonia was detected immunocytochemically with a cell proliferation kit. After 24 h culture, LIF signicantly increased the percentage of labelled prospermatogonia, with the peak of activity at 20 pg mL -1 . The combination of LIF and SGF resulted in a decrease in DNA synthetic activity of the germ cells. Hence, LIF and SGF play a role in local regulation at the onset of spermatogenesis in the rat testis. 
