
%0 Journal Article
%A Kreysing, U. 
%A Nagai, T. 
%A Niemann, H. 
%T Male-dependent variability of fertilization and embryo development in two bovine <emph type="2">in vitro</emph> fertilization systems and the effects of casein phosphopeptides (CPPs)
%J Reproduction, Fertility and Development
%D 1997/11/14
%V 9
%N 4
%P 465-474
%K casein-phosphopeptides, penetration, blastocysts.
%R https://doi.org/10.1071/R96097
%U https://www.publish.csiro.au/paper/R96097 
%X This study investigated the effects of semen from five different bulls and two different ejaculates of the same bull on penetration, cleavage, blastocyst formation, and cell allocation in bovine blastocysts produced in vitro. Casein phosphopeptides (CPPs) were tested for their ability to enhance fertilization and minimize variability among bulls and ejaculates. In Experiment 1, the BO-fertilization system was employed. Penetration and polyspermy both displayed great variation among bulls and between ejaculates, whereas no significant differences were observed in cleavage and blastocyst-formation rates. Similar variability was observed in penetration, polyspermy, cleavage, blastocyst-formation rates and cell allocation and distribution when the two fertilization systems, TALP and BO, were compared in Experiment 2. The BO-system supported penetration and polyspermy better (P &#60; 0&#183;05) than the TALP-system, whereas the TALP-system was superior (P &#60; 0&#183;05) in supporting cleavage and blastocyst formation. Significant interactions existed between bulls and the fertilization system employed. It is concluded that the success of in vitrofertilization is markedly dependent on individual bulls as well as on ejaculates from the same bull. CPPs are able to enhance penetration and embryo development in certain bulls or ejaculates and thus contribute to reducing the degree of individual variability, but they do not generally improve the success of bovine embryo production in vitro. 
