8 SYNCHRONIZATION OF OVULATION IN DAIRY HEIFERS USING A SHORTENED ESTRADIOL-BASED PROTOCOL THAT PROVIDES FOR A LENGTHENED PROESTRUS

Abstract

Several studies, performed mainly in North America, have shown that the 5-day co-synch timed-AI (TAI) protocol results in similar or higher pregnancy rates than conventional 7-day co-synch protocols in beef cows and beef and dairy heifers. It is hypothesised that the 5-day co-synch protocol reduces the incidence of persistent follicles and provides for a longer proestrus, with increasing oestradiol concentrations due to continuous gonadotropin support for the dominant follicle (Bridges et al. 2008 Theriogenology 69, 843–851). Similar findings have been reported recently following the use of a shortened oestradiol-based protocol that also provides for a longer proestrus (named J-synch; de la Matta and Bo 2012 Taurus 55, 17–23). A study was designed to compare these 2 treatment protocols for synchronization of ovulation that allow for a prolonged proestrus with the conventional oestradiol-based protocol currently used for TAI in South America for heifers. Cycling Holstein heifers that were, on average, 17 months of age, weighting 350 to 380 kg, and with a body condition score of 2.5 to 3.5 (scale of 1 to 5), were randomly allocated to 1 of 3 treatment groups. Heifers in the 7-day estradiol benzoate (EB) group received a progesterone device (DIB 1 g of progesterone; Syntex SA, Buenos Aires, Argentina) and 2 mg EB (oestradiol benzoate, Syntex SA) on Day 0, 500 μg of cloprostenol (prostaglandin F_2α; Sincronil, Proagro SA, Argentina) and DIB removal on Day 7, 1 mg of EB on Day 8, and TAI 30 h later (54 h after DIB removal). Heifers in the J-synch group received a DIB and 2 mg of EB on Day 0, PGF and DIB removal on Day 6, and 10 μg of buserelin acetate [gonadotropin-releasing hormone (GnRH); Receptal, Intervet, Millsboro, MD, USA] and TAI on Day 8 (72 h later). Heifers in the 5-day co-synch group received a DIB and GnRH on Day 0, DIB removal and prostaglandin F_2α (twice, 12 h apart) on Day 5, and GnRH along with TAI on Day 8 (72 h later). Treatments were coordinated so that DIB were removed in all groups at the same time to decrease a possible time effect of follicle parameters and pregnancy rates. Two experiments were conducted; the first evaluated follicular dynamics and ovulation rates by twice daily ultrasonography (Honda 101 V, 5.0 MHz) in 20 heifers per group and the second determined pregnancy rates to TAI in 172 heifers. The mean (± standard error of the mean) interval from DIB removal to ovulation was shorter in the 7-day EB group (82.7 ± 3.1 h) than in the J-synch (103.8 ± 3.3 h) and 5-day co-synch (96.8 ± 3.3 h) groups (P = 0.01). However, the mean diameter of the dominant preovulatory follicle was smaller (P = 0.01) in the 7-day EB (12.8 ± 0.5 mm) and the J-synch (13.1 ± 0.6 mm) groups than in the 5-day co-synch group (14.9 ± 0.6 mm). Although pregnancy rates in the second experiment were numerically lower in the 7-day EB group (31/58; 53.4%) and 5-day co-synch group (30/55; 54.5%) than in the J-synch group (37/59; 62.7%), differences were not significant (P > 0.3). Preliminary results indicate that the 3 protocols evaluated result in comparable pregnancy rates to TAI in dairy heifers.