228 RECIPIENT PREPARATION FOR SPERMATOGONIAL STEM CELL TRANSPLANTATION: ALTERATION IN TESTICULAR CELL COMPONENTS FOLLOWING TRANSIENTLY INDUCED ISCHEMIA
G. M. Schuenemann A , S. M. L. C. Mendis-Handagama B , T. M. Prado B , H. S. Adair B and F. N. Schrick AA Department of Animal Science, Tennessee Agricultural Experiment Station, The University of Tennessee, Knoxville, TN, USA;
B College of Veterinary Medicine, The University of Tennessee, Knoxville, TN, USA
Reproduction, Fertility and Development 20(1) 193-194 https://doi.org/10.1071/RDv20n1Ab228
Published: 12 December 2007
Abstract
Depletion of endogenous spermatogonial stem cells using busulfan (Brinster et al. 2003 Biol. Reprod. 69, 412–420) or irradiation (Izadyar et al. 2003 Reproduction 126, 765–774) have been used in preparation of recipient animals prior to transplantation; however, both techniques are not without compromises (severe bone marrow depression or specialized radiotherapy equipment required). Induced testicular ischemia in rams altered spermatic epithelium with germ cell-depleted seminiferous tubules (Markey et al. 1994 Reprod. Fertil. 101, 643–650). The objective was to evaluate testicular transiently induced ischemia (using elastrator bands) in Jersey calves on testicular morphology and development. Treatments (at 27 ± 5 days of age) consisted of control (0, n = 4), banding for periods of 2 h (2, n = 4), 4 h (4, n = 4), and 8 h (8, n = 4). After castration (age: 60 ± 5 days), the right testis of each animal was used for calculation of cell components per testis according to the point counting method. Data were analyzed using the MIXED procedure of SAS (SAS Institute, Inc., Cary, NC, USA). Body weight (59.8 ± 6.2 kg) and scrotal circumference (SC) at banding (9.1 ± 0.2 cm) did not differ between treatments. Fresh testis weight (TW), scrotal temperature immediately before banding removal (ST), and daily scrotal circumference growth (SC) were decreased (4 and 8 h) in ischemic testes compared to controls (Table 1: P < 0.05). In addition, Sertoli and Leydig cells were severely reduced in the 8-h ischemic treatment (Table 1: P < 0.05). Transiently induced ischemia significantly decreased the number of germ cells in the 8-h group, compared to the 0-, 2-, and 4-h groups (Table 1: P < 0.05). These results suggest that transiently induced ischemia significantly decreases the number of germ, Sertoli, and Leydig cells in the testis. Therefore, these present findings could be applicable for preparation of recipient animals through depletion of endogenous germ cells within the seminiferous tubules. This procedure may provide a suitable environment for transplanted donor germ cell colonization in prepubertal recipient bulls.
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