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Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

215 PRODUCTION OF PROGENY WITH SEXED WHITE HANWOO (KOREAN NATIVE ALBINO CATTLE) SEMEN

J. S. Choi A , C. Y. Choe A , D. K. Kim A , H. H. Seong A and S. W. Kim A
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- Author Affiliations

Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon, Korea

Reproduction, Fertility and Development 28(2) 238-239 https://doi.org/10.1071/RDv28n2Ab215
Published: 3 December 2015

Abstract

The aim of the study was to produce sexed progeny of the genetically confined White Hanwoo (albinism). A White Hanwoo bull was selected as the semen donor. The X-bearing sperm were sorted by MoFlo XDP cell sorter from Korea Sexing Biotech (Daegu, South Korea). To compare pregnancy and birth rates as well as semen quality, a Korean proven bull (KPN) was used as control. Total number of nonsorted sperm was 20 × 106 per straw. Double insemination of 2 or 4 × 106 sexed and frozen X-bearing sperm were performed on Hanwoo heifers. The abnormality of nonsorted albino spermatozoa (5 ejaculates) was 24.9 ± 7.31% (but 18.7 ± 6.2% C in KPN 768, derived from 5 straws) with a higher number of distal reflex abnormality in midpiece (11.7% compared with that of KPN 768, 5.6%). There were no differences in pregnancy and birth rates when 2 or 4 × 106 X-bearing sperm were inseminated but nonsorted semen had higher pregnancy rates (P < 0.05). The pregnancy rates of KPN 768, 2 × 106 cells, and 4 × 106 cells X-bearing sperm of White Hanwoo cattle were 85.0% (17/20), 26.3% (5/19), and 50% (7/14), respectively. The birth rates were 80.0% (16/20), 15.8% (3/19), and 21.4% (3/14), respectively. The female offspring rates were 43.8% (7/16), 100% (3/3), and 100% (3/3) (P < 0.05). These results indicated that sex-sorted albino Hanwoo semen could be used for the production of wanted progeny with 2 × 106 cells/straw for AI to heifers.