46 EFFECT OF MANIPULATION MEDIUM ON THE DEVELOPMENT OF RECONSTRUCTED DOMESTIC CAT EMBRYOS
S. Imsoonthornruksa A , C. Lorthongpanich A , K. Srirattana A , N. Sripunya A , C. Laowtammathron A , M. Ketudat-Cairns A and R. Parnpai AAEmbryo Technology and Stem Cell Research Center, Suranaree University of Technology, Nakhon Ratchasima, Thailand 30000
Reproduction, Fertility and Development 19(1) 141-141 https://doi.org/10.1071/RDv19n1Ab46
Submitted: 12 October 2006 Accepted: 12 October 2006 Published: 12 December 2006
Abstract
Domestic cat can serve as a valuable model for assisted reproductive techniques studies of endangered felid species. Therefore, this study was conducted to examine the effect of different manipulation medium on in vitro development of reconstructed domestic cat oocytes. The oocytes were recovered by slicing the ovaries of the cats that had been superstimulated with 200 IU eCG (Intervet, Boxmeer, The Netherlands). The procedures for SCNT were described previously (Lorthongpanich et al. 2004 Reprod. Fertil. Dev. 16, 149 abst). The manipulation medium for SCNT procedures was evaluated between HEPES-buffered TCM-199 (Sigma-Aldrich Corp., St Louis, MO, USA) + 10% FBS (199H) and Emcare embryo holding solution (ICPbio, Ltd., Auckland, New Zealand) (Emcare) during the denuding, enucleation, injection, activation, and holding steps. Parthenogenetic activation (PA) embryos were used as a control for both media. There was no significant difference in fusion rate when either 199H (63%) or Emcare (76%) was used. The cleavage, 8-cell, and morulae development rates of SCNT and PA were not significantly different when using either 199H or Emcare (Table 1). However, the blastocyst formation rates of SCNT and PA in Emcare (44% and 29%, respectively) were significantly greater than those of 199H (14% and 18%, respectively; P < 0.05). These results indicated that the manipulation medium is important for SCNT blastocyst development.
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