137 Comparison of two in vitro maturation media on polar body extrusion of cattle oocytes
S. M. Sithole A B , M. L. Mphaphathi A C , M. D. Sebopela A B and T. L. Nedambale A BA Germplasm Conservation & Reproductive Biotechnologies, Animal Production, Agricultural Research Council, Irene, South Africa;
B Department of Animal Sciences, Faculty of Science, Tshwane University of Technology, Pretoria, South Africa;
C Department of Animal, Wildlife and Grassland Sciences, University of the Free State, Bloemfontein, South Africa
Reproduction, Fertility and Development 33(2) 176-177 https://doi.org/10.1071/RDv33n2Ab137
Published: 8 January 2021
Abstract
The in vitro embryo production technique is one of the assisted reproduction technologies that has the potential in speeding up genetic improvement in cattle. The developmental competence of in vitro-matured oocytes is influenced by several factors during in vitro maturation (IVM), such as maturation environment, oocyte quality, type of media, and additives. The objective of the present study was to compare two IVM media (TCM-199 and BO-IVM) on cattle oocyte maturation rate in vitro. Cattle ovaries were collected from local slaughterhouse and transported to the laboratory in a thermos flask containing 0.9% saline (Adcock Ingram Critical care) at 37°C. Oocytes were retrieved from the ovaries by the aspiration technique, and then matured in vitro in 500 µL of TCM-199 (supplemented with 10% fetal bovine serum, FSH, LH, and E2) or in 500 µL of commercially available BO- IVM (Bioscience) medium, both covered with mineral oil for 22 h at 38.5°C with 5% CO2 and 5% O2. After 22 h of IVM, oocyte polar body extrusion was evaluated with the aid of Oosight Imaging System (Hamilton Thorne) connected to an inverted microscope. The total number of oocytes matured in TCM-199 and BO-IVM media were 401 and 396, respectively. The experiment was replicated 19 times. Data were analysed using the GenStat® program (VSN International). Means of different treatments were separated using Fisher’s protected t-test least significant difference at 5% level of significance. No difference was recorded for polar body extrusion rates in TCM-199 (50.6 ± 13.9) and BO-IVM (47.3 ± 13.7) media. In conclusion, TCM-199 and BO-IVM media did not differ in terms of maturation rate; thus, both can be used for successful cattle oocyte IVM.
